2010 Fiscal Year Final Research Report

Study of rejuvenation of aged oocyte "Aim at the establishment of the strategy of a new fertility treatment"

Research Project

Project/Area Number	20591905
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Yamagata University
Principal Investigator	IGARASHI Hideki Yamagata University, 医学部, 助教 (80333970)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Toshifumi 山形大学, 医学部, 講師 (20302292)
Project Period (FY)	2008 – 2010
Keywords	生殖医学
Research Abstract	Mitochondria (Mt) extracted from the hepatocyte to the purpose of the rejuvenation of the aged mouse were transplanted to ooplasm. However, neither the fertilization rate nor the embryonic development of the Mt transplant aged oocytes have been improved. The endoplasmic reticulum (ER) stress was examined as a new mechanism of aging of oocytes. An increase of the ER stress marker was confirmed in the aged mouse oocytes. The ER stress inducement material, thapsigargin, and tunicamycin decreased the fertilization rate after the in vitro fertilization of fresh mouse oocytes, and worsened the embryonic development. As for the above-mentioned result, the ER stress takes part in the aging of the oocyte, and overcoming the ER stress suggests that it be important in the rejuvenation of the aged oocyte.

(5 results)