2010 Fiscal Year Final Research Report
Study on the expression of ion channels in inflammation-induced uterine smooth muscle elucidating the mechanisms of preterm delivery.
| Project/Area Number |
20591917
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMAOKA Kaoru Hiroshima University, 保健医療学部, 教授 (10200586)
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| Co-Investigator(Kenkyū-buntansha) |
MIYOSHI Hiroshi 広島国際大学 (40294590)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ATP受容体 / P2X7チャンネル / 子宮平滑筋 / 早産 / 周産期医学 / パッチクランプ |
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| Research Abstract |
In pregnant rat myometrium, P2X4 and P2X7 receptors, one of ATP-sensitive non-selective cation channels (NSCC), were suggested to be highly expressed by the quantitative evaluation of mRNA s and proteins. NSCC currents observed in freshly isolated cells from pregnant rat myometrium resembles to those from COS7 cells where P2X7 receptors were transfected, indicating P2X7 receptor in rat myometrium play a major role in conducting NSCC currents. It may be suggested that C^<2+> influx through P2X7 may trigger labor contraction. In uterus of inflammatory model rats induced by application of LPS (lipopolysaccharide), P2X7 receptors were highly expressed. Thus, these results indicate that stimulation of P2X7 receptor expression in rat myometrium induced by inflammation may lead to preterm delivery. Further study on this subject should contribute to the elucidation of precise mechanisms of preterm delivery as well as development of new therapies and new technologies of prevention against preterm delivery.
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