Anti-aging research on the oocyte reproductive function based on the gene expression profiling

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591929
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: SUEOKA Kou Keio University, 医学部, 准教授 (90162833)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Yasunori 慶應義塾大学, 医学部, 教授 (10129736) ; HASHIBA Tsuyoshi 慶應義塾大学, 医学部, 助教 (60245553)
• Project Period (FY): 2008 – 2010
• Keywords: 遺伝子発現プロファイリング / アポトーシス / ミトコンドリア / 加齢変化 / 胚

Research Abstract

Mitochondria exists in cell as an organelle that takes part in the role of oxidative phosphorylation to supply ATP to the cell. Our studies demonstrated that lower mtDNA copy number existed in unfertilized oocytes and uncleavaged embryos originated from over 40 years of age women significantly. A positive correlation was demonstrated between mtDNA copy number and the cell volume of blastomere. We examined the quality of embryo in zebrafish, in terms of the implication between morphological classification of embryo and embryonic development. Glowing of poor form embryo was delayed and the efficiency of embryonic development tended to be reduced by aging. From the respect of apoptosis on the oocyte, microarray analysis was applied to caspase gene expression in zebrafish. Embryo was revealed in apoptosis through the increased expression of caspase genes.

Research Products

• [Presentation] 卵子ミトコンドリアDNA copy数が受精能・胚発生能へ与える影響 (2009)
  • Author(s): 村越行高,末岡浩,高橋香織,佐藤卓,櫻井友義,渡邊広是,田島博人,佐藤健二,中林章,大澤淑子,橋場剛士,久慈直昭,吉村泰典
  • Organizer: 第54回日本生殖医学会
  • Place of Presentation: 石川県立音楽堂(金沢市)
  • Year and Date: 2009-11-23
• [Presentation] 卵子,顆粒膜細胞のミトコンドリアDNA copy 数・変異が受精能・胚発生能へ及ぼす影響 (2009)
  • Author(s): 村越行高,末岡浩,佐藤卓,櫻井友義,渡邊広是,田島博人,中林章,浅田弘法,橋場剛士,久慈直昭,吉村泰典
  • Organizer: 第50回日本哺乳動物卵子学会都市センターホテル
  • Place of Presentation: 東京都
  • Year and Date: 2009-05-08
• [Presentation] ゼブラフィッシュ胚を用いた形態と分割速度による胚の分類とその検討 (2008)
  • Author(s): 櫻井友義,末岡浩,佐藤卓,吉村泰典,中林章,田島博人,渡邊広是
  • Organizer: 第26回日本受精着床学会学術講演会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2008-08-28
• [Presentation] 卵子、顆粒膜細胞におけるミトコンドリアDNAの加齢因子が受精能・胚発生能へ及ぼす影響 (2008)
  • Author(s): 村越行高,末岡浩,佐藤卓,櫻井友義,渡邊広是,田島博人,中林章,橋場剛士,岩田壮吉,久慈直昭,青木大輔,吉村泰典
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2008-04-12