2010 Fiscal Year Final Research Report
The expression of mammalian tumor suppressor, Int6 and hypoxia inducible factor(HIF)-2α in ovarian carcinomas
| Project/Area Number |
20591941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shinshu University |
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Principal Investigator |
OHIRA Satoshi Shinshu University, 医学部附属病院, 助教 (90397315)
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| Co-Investigator(Kenkyū-buntansha) |
OSADA Ryousuke 信州大学, 医学部附属病院, 助教 (80418722)
HORIUCHI Akiko 信州大学, 医学部, 講師 (80334895)
SHIOZAWA Tanri 信州大学, 医学部, 教授 (20235493)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 卵巣癌 / Int6 / HIF / VHL |
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| Research Abstract |
In order to elucidate the possible involvement of Int6 in ovarian carcinomas, we examined the immunohistochemical expression of Int6, HIF-2α and von Hippel-Lindau (VHL) in ovarian carcinomas, and analyzed the prognostic differences according to Int6, HIF-2α and VHL expression in ovarian carcinoma patients. We examined the immunohistochemical expression of Int6, HIF-2α, and VHL in 70 cases of ovarian carcinomas. In addition, we analyzed the correlation and prognostic differences according to the expression of Int6, HIF-2α and VHL. We found that Int6 and VHL protein were expressed in the cytoplasm of tumor cells. Although the HIF-2α staining was mainly observed in the cytoplasm, nuclear staining was sporadically observed in ovarian carcinoma cells. Cytoplasmic expression of HIF-2α was significantly higher in tumors of FIGO stage III+IV than those of stage I+II. In advanced cases, HIF-2α expression showed inverse correlation with the expression of Int6. There was no significant correlation between HIF-2α and VHL expression. The log-rank test showed that the cytoplasmic positive HIF-2α immunostaining in tumor cells is associated with the poor prognosis of ovarian carcinoma patients (p=0.005). Interestingly, the prognosis was significantly poorer in those with positive HIF-2α and negative Int6 immunostaining. These results show that the increased expression of HIF-2α subunit and decreased expression of Int6 may involved in the progression of ovarian carcinoma.
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Research Products
(25 results)
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[Journal Article] Oct4 expression in immature teratoma of the ovary : relevance to histologic grade and degree of differentiation.2010
Author(s)
Abiko K, Mandai M, Hamanishi J, Matsumura N, Baba T, Horiuchi A, Mikami Y, Yoshioka S, Wakasa T, Shiozawa T, Konishi I.
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Journal Title
Am J Surg Pathol. 34(12)
Pages: 1842-1848
Peer Reviewed
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[Journal Article] Distinguishing primary from secondary mucinous ovarian tumors : an algorithm using the novel marker DPEP1.2010
Author(s)
Okamoto T, Matsumura N, Mandai M, Oura T, Yamanishi Y, Horiuchi A, Hamanishi J, Baba T, Koshiyama M, Shiozawa T, Konishi I.
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Journal Title
Peer Reviewed
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[Journal Article] Outcomes of fertility-sparing surgery for stage I epithelial ovarian cancer : a proposal for patient selection.2010
Author(s)
Satoh T, Hatae M, Watanabe Y, Yaegashi N, Ishiko 0, Kodama S, Yamaguchi S, Ochiai K, Takano M, Yokota H, Kawakami Y, Nishimura S, Ogishima D, Nakagawa S, Kobayashi H, Shiozawa T, Nakanishi T, Kamura T, Konishi I, Yoshikawa H
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Journal Title
J Clin Oncol. 28(10)
Pages: 1727-1732
Peer Reviewed
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[Journal Article] Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma : the important role of Snail in ovarian tumorigenesis and progression.2009
Author(s)
Yoshida J, Horiuchi A, Kikuchi N, Hayashi A, Osada R, Ohira S, Shiozawa T, Konishi I.
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Journal Title
Med Mol Morphol. 42(2)
Pages: 82-91
Peer Reviewed
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