2010 Fiscal Year Final Research Report
Experimental study on new treatment strategy of retinoblastoma
| Project/Area Number |
20592055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
MIYAZAKI Kenichi Wakayama Medical University, 医学部, 博士研究員 (40382329)
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| Co-Investigator(Kenkyū-buntansha) |
SAIKA Shizuya 和歌山県立医科大学, 医学部, 教授 (40254544)
OKADA Yuka 和歌山県立医科大学, 医学部, 講師 (50264891)
TOMOYOSE Katsuo 和歌山県立医科大学, 医学部, 助教 (00453176)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 小児悪性腫瘍 / Sonic hedgehog / Smad / Y79 / 網膜芽細胞腫 |
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| Research Abstract |
The study was conducted for the purpose of development of a new strategy of treatment of retinoblastoma by targeting Sonic Hedgehog (Shh) signal. First, immunohistochemistry was carried out to examine the status of Shh signal and cell-growth related signals. Shh, Smoothened Patched and Gli-1 weere readily detected in retinoblastoma cells. Rb and Erk-1 was also observed. These findings suggest that Shh signal is active in retinoblastoma cells.
A culture of a cell line established from retinoblastoma (Y79) was treated with cyclopamine and cell proliferation was assayed by using MTT assay. Although adding cyclopamine seemed to suppress cell proliferation of Y79 retinoblastome cell line, the data did not exhibit statistical significacy.
Neovascularization is critical in tumor growth. We showed that blocking Shh signal by addition of cyclopamine inhibited neovascularization by cultured vascular endothelial cells cultured on the fibroblast layer.
Finally, we implanted Y79 cells into the anterior chamber of a C57BL/6 mouse. However, the cells did not form tumor growth and settled arounf the anterior chamber angle. Further improvement
of cell implantation is to be developed for the purpose of establishment of retinoblastoma mouse
model.
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Research Products
(16 results)
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[Journal Article] Corneal wound healing in an osteopontin-deficient mouse2008
Author(s)
Miyazaki K, Okada Y, Yamanaka O, Kitano A, Ikeda K, Kon S, Uede T, Rittling SR, Denhardt DT, Kao WW, Saika S
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Journal Title
Invest Ophthalmol Vis Sci. 49(4)
Pages: 1367-75
Peer Reviewed
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[Presentation] 実験的脈絡膜線維血管増生モデルを用いた研究2008
Author(s)
宮崎賢一, 山中修, 藤田恭子, 藤田周子、石川伸之, 北野愛, 友寄勝夫, 住岡孝吉, 宮本武, 田中才一, 岡田由香, 雑賀司珠也
Organizer
第112回日本眼科学会総会
Place of Presentation
横浜
Year and Date
2008-04-18
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