2010 Fiscal Year Final Research Report
Research on regenerative medicine of pediatric airways using new materials as scaffolds and scaffold-free cell sheets.
| Project/Area Number |
20592087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
USUI Noriaki Osaka University, 医学系研究科, 准教授 (30273626)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Gakuto 大阪大学, 医学部附属病院, 医員 (60467561)
SAWAI Toshio 大阪大学, 医学系研究科, 助教 (90351808)
WATANABE Jyunji 大阪大学, 工学系研究科, 准教授 (60323531)
KAMIYAMA Masafumi 大阪大学, 医学系研究科, 助教 (20403074)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 再生医療 / 気管軟骨 / Scaffold-free / ポリトリメチレンカーボネイト / Tissue engineering / Cell sheet / 回転培養 / Cylindrical cartilage |
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| Research Abstract |
We first tried to use Poly-tri-methylene-carbonate as new materials for scaffolds in cartilage regeneration, however resulted in failure of regeneration. Then, we developed a novel procedure for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. Auricular chondrocytes were harvested from rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks. The engineered cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation.
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