2010 Fiscal Year Final Research Report
Molecular regulation governing epithelio-myogenic cell interactions during early development of mouse tongue
| Project/Area Number |
20592157
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | The Nippon Dental University |
|---|
Principal Investigator |
TAYA Yuji The Nippon Dental University, 生命歯学部, 准教授 (30197587)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SOENO Yuuichi 日本歯科大学, 生命歯学部, 講師 (70350139)
SHIMADZU Yoshihito 日本歯科大学, 生命歯学部, 講師 (10297947)
AOBA Takaaki 日本歯科大学, 生命歯学部, 教授 (30028807)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 歯学 / 病理学 / 舌発生 / 上皮間葉相互作用 / 筋分化 / 遺伝子発現 |
|---|
| Research Abstract |
We herein aimed to investigate the timing and positional regulation mechanism of myogenic lineage differentiation through epithelial-myogenic precursor interactions during the development of lateral lingual swellings (LLS). Microarray, GO and pathway analyses unveiled that a number of genes, which were assigned mostly to Shh, Fgf, Wnt and Tgfb signaling pathways, were upregulated in a region-specific manner. The organ culture of mouse branchial arches was proved to assimilate satisfactorily the myogenic-lineage differentiation and the morphologic characteristics of the LLS. Administration of rhFgf8 (25 ng/mL) to the culture medium was sufficient to mediate an evasion of cell death in LLS. Also the treatment with Shh inhibitor (Jervine 5μM) brought about interference of mesenchymal cell proliferation and aberrant localization of myogenic-lineage cells, resulting in microglossia.
|
