2010 Fiscal Year Final Research Report
Role(s) of pepstatin A-sensitive aspartic proteinases in osteoclast differentiation
| Project/Area Number |
20592175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
NISHISHITA Kazuhisa Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (20237697)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKUBA Takayuki 長崎大学, 大学院・医歯薬学総合研究科, 教授 (30264055)
OKAMOTO Kuniaki 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (10311846)
SAKAI Eiko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10176612)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 破骨細胞 / プロテアーゼ / カテプシンE / カテプシンD / アポトーシス / ナプシン |
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| Research Abstract |
We investigated the roles of aspartic proteinase family in mouse in vitro osteoclast differentiation model. Osteoclastogenesis in bone marrow macrophage culture from cathepsin E-knockout mice were significantly suppressed, probably through elevating the RANKL-induced caspase 3 activation. We also studied the comparative genetic and biochemical analyses of napsins. Napsin A is endogenously expressed in mouse bone marrow cells and recombinant proteins possess pepstatin-A sensitive activities against several synthetic aspartic proteinase substrates.
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