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2010 Fiscal Year Final Research Report

Gene therapy using sonoporation for maxillofacial distraction

Research Project

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Project/Area Number 20592340
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKyushu Dental College

Principal Investigator

YOSHIOKA Izumi  Kyushu Dental College, 医学部, 准教授 (10305823)

Co-Investigator(Kenkyū-buntansha) SAKODA Sumio  宮崎大学, 医学部, 教授 (20118079)
IGAWA Kaori  宮崎大学, 医学部, 助教 (90423722)
Project Period (FY) 2008 – 2010
Keywords顎骨延長法 / BMP
Research Abstract

Bone morphogenetic protein-2 (BMP-2) is expected to be utilized to fill bone defects and promote healing of fractures. However, it is unable to generate an adequate clinical response for use in bone regeneration. Recently, it was reported that glycosaminoglycans, including heparin, heparan sulfate, keratan sulfate, dermatan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid (HA), regulate BMP-2 activity, though the mechanism by which HA regulates osteogenic activities has not been fully elucidated. The aim of this study was to investigate the effects of HA on osteoblast differentiation induced by BMP-2.Monolayer cultures of osteoblastic lineage MG63 cells were incubated with BMP-2 and HA for various time periods. To determine osteoblastic differentiation, alkaline phosphatase (ALP) activity in the cell lysates was quantified. Phosphorylation of Smad 1/5/8, p38, and ERK proteins was determined by Western blot analysis. To elucidate the nuclear translocation of phosphorylated Smad 1/5/8, stimulated cells were subjected to immunofluorescence microscopy. To further elucidate the role of HA in enhancement of BMP-2-induced Smad signaling, mRNA expressions of the BMP-2 receptor antagonists noggin and follistatin were detected using real-time RT-PCR.BMP-2-induced ALP activation, Smad 1/5/8 phosphorylation, and nuclear translocation were up-regulated when MG63 cells were cultured with both BMP-2 and HA. Western blot analysis revealed that phosphorylation of ERK protein was diminished by HA. Furthermore, the mRNA expressions of noggin and follistatin induced by BMP-2 were preferentially blocked by HA.These results indicate that HA enhanced BMP-2 induces osteoblastic differentiation in MG63 cells via down-regulation of BMP-2 antagonists and ERK phosphorylation.

  • Research Products

    (10 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) Presentation (2 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Mechanism involved in enhancement of osteoblast differentiation by hyaluronic acid.2011

    • Author(s)
      Michinao Kawano, Wataru Ariyoshi, Kenjiro Iwanaga, Toshinori Okinaga, Manabu Habu, Izumi Yoshioka, Kazuhiro Tominaga
    • Journal Title

      Biochemical and Biophysical Research Communications 25

      Pages: 575-580

  • [Journal Article] Relationship Between Inferior Alveolar Nerve Canal Position at Mandibular Second Molar in Patients With Prognathism and Possible Occurrence of Neurosensory2010

    • Author(s)
      Yoshioka I, Tanaka T, Khanal A, Habu M, Kito S, Kodama M, Oda M, Wakasugi-Sato N, Matsumoto-Takeda S, Fukai Y, Tokitsu T, Tomikawa M, Seta Y, Tominaga K, Morimoto Y
    • Journal Title

      Disturbance After Sagittal Split Ramus Osteotomy J Oral Maxillofac Surg. 68

      Pages: 3022-3027

  • [Journal Article] ラット下顎骨延長法におけるSmadの動態2010

    • Author(s)
      山中雅博、吉岡泉、土生学、冨永和宏
    • Journal Title

      九州歯科会誌 63

      Pages: 268-276

  • [Journal Article] Post-operative skeletal stability and accuracy of a new combined Le Fort I and horseshoe osteotomy for superior repositioning of the maxilla2009

    • Author(s)
      I.Yoshioka,, A.Khanal, N.Furuta, M.Kodama, K.Tominaga
    • Journal Title

      Int J Oral Maxillofac Surg 38

      Pages: 1250-1255

  • [Journal Article] Maxillary reconstruction using an angular branch of scapula2009

    • Author(s)
      I.Yoshioka, Y.Yamashita, A.Khanal, M.Kodama, T.Takahashi, K.Tominaga
    • Journal Title

      Int J Oral Maxillofac Surg 38

      Pages: 1311-1315

  • [Journal Article] Effects of latency on bone formation during rabbit mandibular distraction osteogenesis.2008

    • Author(s)
      Kazuoki Hayashi、Izumi Yoshioka、Norihiko Furuta、Amit Khanal、Kazuhiro Tominaga
    • Journal Title

      Asian Journal of Oral and Maxillofac Surg 20

      Pages: 5-11

  • [Presentation] ポリグリコール酸シートとフィブリン糊スプレーを用いた創面被覆法の使用経験2010

    • Author(s)
      吉岡泉、井川加織、高森晃一、永田順子、鹿嶋光司、迫田隅男
    • Organizer
      第55回(社)日本口腔外科学会総会・学術集会
    • Place of Presentation
      千葉市
    • Year and Date
      20101016-20101018
  • [Presentation] 歌舞伎メーキャップ症候群に伴った唇顎口蓋裂を有する骨格性反対咬合の1例2010

    • Author(s)
      吉岡泉、副島和久、井川加織、鹿嶋光司、迫田隅男
    • Organizer
      第20回特定非営利活動法人日本顎変形症学会総会
    • Place of Presentation
      札幌市
    • Year and Date
      20100615-20100616
  • [Book] よくわかる歯科医学・口腔ケア医学情報社(喜久田利弘楠川仁悟編集)(歯と歯周組織の発生と解剖)2011

    • Author(s)
      吉岡泉
    • Total Pages
      10-11
    • Publisher
      医学情報社
  • [Remarks] ホームページ等

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Published: 2012-01-26   Modified: 2016-04-21  

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