2010 Fiscal Year Final Research Report
Study of natural inducer for coral larval settlement and metamorphosis.
| Project/Area Number |
20611003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Keio University |
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Principal Investigator |
KITAMURA Makoto Keio University, 医学部, 助教 (00436990)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 造礁サンゴ / 幼生 / 変態誘引物質 / 無節サンゴモ |
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| Research Abstract |
A new metamorphosis-enhancing macrodiolide, luminaolide, was isolated from the crustose coralline algae (CCA) Hydrolithon reinboldii. Its structure was determined by spectroscopic analysis. A fraction (1.30 μg/mL) eluted with 80% aqueous MeOH by ODS gel column chromatography of the same CCA extract induced larval metamorphosis (25.9±7.4%) against Leptastrea purpurea, and its metamorphosis-inducing activity was further enhanced to 92.6±2.9% with the addition of 1 (25.6 ng/mL).
Furthermore, eight bromotyrosine derivatives including 11-deoxyfistularine-3 were prepared and tested for their metamorphosis-inducing activity against larvae of scleractinian coral Pseudosiderastrea tayamai. The following structure-activity relationships were noted : (i) two spiroisoxazolin moieties are essential, tyramine derivetive between both spiroisoxazolin moieties is not essential for the activity. (ii) Two hydroxyl groups in each spiroisoxazolin moiety are important for the activity.
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