2012 Fiscal Year Final Research Report
Mechanisms for methylation imprinting establishment afterfertilization
| Project/Area Number |
20678002
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| Research Category |
Grant-in-Aid for Young Scientists (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2012
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| Keywords | 発現制御 / エピジェネティクス |
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| Research Abstract |
enomic imprinting is an epigenetic phenomenon in mammals, in which a subset of genes are mono-allelically expressed depending on their parental origin.To reveal identity of the epigenetic signature discriminating the parental origin and its deposition site within the H19-ICR sequence, transgenic mouse methodology was employed and following findings were demonstrated:
1) Paternal H19-ICR acquired methylation independently of its establishment in sperm
2) CTCF sites were not required to establish paternal-allele-specific methylation in pre-implantation embryos
3) Sequences in the H19-ICR corresponding to the small RNA sequences in oocytes were dispensable for methylation imprinting
4) Differential methylation of the H19-ICR was established by its paternal-allele-specific methylation and maternal-allele-specific protection-against-methylation
5) Oct-sox motif in the H19-ICR harbored protection-against-methylation activity in vivo after implantation
6) Chicken HS4 insulator did not protect the H19-ICR from differential DNA methylation.
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Research Products
(11 results)
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[Journal Article] The H19 ICR mediates pre-implantation imprinted methylation of nearby sequences in YAC transgenic mice2013
Author(s)
Okamura, E., Matsuzaki, H., Sakaguchi, R., Takahashi, T., Fukamizu, A. and Tanimoto, K
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Journal Title
Mol. Cell. Biol.
Volume: 334
Pages: 858-71
DOI
Peer Reviewed
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[Journal Article] Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting inYAC transgenic mice2012
Author(s)
Takahashi, T., Matsuzaki, H., Tomizawa,S.-i., Okamura, E., Ichiyanagi, T., Fukamizu, A., Sasaki, H. and Tanimoto, K.
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Journal Title
Gene
Volume: 508
Pages: 26-34
DOI
Peer Reviewed
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