Multiple layers of regulatory machineries for actin cytoskeleton at the leading edge

2010 Fiscal Year Final Research Report

• Project/Area Number: 20687012
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: SUETSUGU Shiro The University of Tokyo, 分子細胞生物学研究所, 准教授 (70345031)
• Project Period (FY): 2008 – 2010
• Keywords: 細胞骨格 / 細胞膜 / 細胞運動 / 細胞内情報伝達 / 浸潤

Research Abstract

The I-BAR domain of IRSp53 deforms plasma membrane into protrusion through its binding of its convex surface to the inner surface of tubular protrusions. In contrast, the F-BAR domain of pacsin2 deforms plasma membrane at the neck of the protrusions, where orthogonal membrane curvature is formed by the concave surface of the F-BAR domain. These two modes of plasma membrane deformation are thought to be important for cellular protrusions including podosome for cancer cell invasion.

Research Products

• [Journal Article] Essential role of PACSIN2/syndapin-II in caveolae membrane sculpting. (2011)
  • Author(s): Senju, Y., Itoh, Y., Takano, K., Hamada, S., Suetsugu, S.
  • Journal Title: J Cell Sci 124(Pt 12) Pages: 2032-2040
• [Journal Article] Phosphoinositide binding by par-3 involved in par-3 localization. (2011)
  • Author(s): Horikoshi, Y., Hamada, S., Ohno, S., Suetsugu, S.
  • Journal Title: Cell Struct Funct 36(1) Pages: 97-102
• [Journal Article] Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation. (2010)
  • Author(s): Takano, K., Watanabe-Takano, H., Suetsugu, S., Kurita, S., Tsujita, K., Kimura, S., Karatsu, T., Takenawa, T., Endo, T.
  • Journal Title: Science 330(6010) Pages: 1536-1540
• [Journal Article] Subcellular membrane curvature mediated by the BAR domain superfamily proteins. (2010)
  • Author(s): Suetsugu, S., Toyooka, K., Senju, Y.
  • Journal Title: Semin Cell Dev Biol 21(4) Pages: 340-349
• [Journal Article] The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins. (2010)
  • Author(s): Suetsugu, S.
  • Journal Title: J Biochem 148(1) Pages: 1-12
• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II. (2010)
  • Author(s): Shimada, A., Takano, K., Shirouzu, M., Hanawa-Suetsugu, K., Terada, T., Toyooka, K., Umehara, T., Yamamoto, M., Yokoyama, S., Suetsugu, S.
  • Journal Title: FEBS Lett 584(6) Pages: 1111-1118
• [Journal Article] Mammalian formin fhod3 regulates actin assembly and sarcomere organization in striated muscles. (2009)
  • Author(s): Taniguchi, K., Takeya, R., Suetsugu, S., Kan, O.M., Narusawa, M., Shiose, A., Tominaga, R., Sumimoto, H.
  • Journal Title: J Biol Chem 284(43) Pages: 29873-29881
• [Journal Article] The direction of actin polymerization for vesicle fission suggested from membranes tubulated by the EFC/F-BAR domain protein FBP17. (2009)
  • Author(s): Suetsugu, S.
  • Journal Title: FEBS Lett 583(21) Pages: 3401-3404
• [Journal Article] EFC/F-BAR proteins and the N-WASP-WIP complex induce membrane curvature-dependent actin polymerization. (2008)
  • Author(s): Takano, K., Toyooka, K., Suetsugu, S.
  • Journal Title: EMBO J 27(21) Pages: 2817-2828
• [Journal Article] Molecules that regulate morphology of the plasma membrane. (2008)
  • Author(s): Suetsugu, S., Takano, K.
  • Journal Title: Tanpakushitsu Kakusan Koso 53(11) Pages: 1326-1336
• [Journal Article] IRSp53 : crossing the road of membrane and actin dynamics in the formation of membrane protrusions. (2008)
  • Author(s): Scita, G., Confalonieri, S., Lappalainen, P., Suetsugu, S.
  • Journal Title: Trends Cell Biol 18(2) Pages: 52-60
• [Journal Article] G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers. (2008)
  • Author(s): Higashida, C., Suetsugu, S., Tsuji, T., Monypenny, J., Narumiya, S., Watanabe, N.
  • Journal Title: J Cell Sci 121(Pt 20) Pages: 3403-3412
• [Presentation] 細胞の形態形成における脂質膜とアクチン細胞骨格の形態制御機構 (2010)
  • Organizer: 定量生物学の会 第3回年会
  • Place of Presentation: 東京大学駒場IIキャンパス
  • Year and Date: 2010-11-27
• [Presentation] Dynamics of actin filaments for subcellular membrane structures (2010)
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2010-09-21
• [Presentation] カベオラにおけるpacsin2/Syndapin IIの機能 (2010)
  • Organizer: 第62回日本細胞生物学会年会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)
  • Year and Date: 2010-05-20
• [Presentation] Membrane tubulation and actin polymerization induced by the EFC/F-BAR domain protein pacsin2 for caveolae formation (2009)
  • Organizer: the 49th Annual Meeting of the American
  • Place of Presentation: Society for Cell Biology (San Diego)
  • Year and Date: 2009-12-07
• [Presentation] The EFC/F-BAR protein that is involved in caveolae formation (2009)
  • Organizer: The second conference on F-BAR proteins
  • Place of Presentation: ストックホルム
  • Year and Date: 2009-10-02
• [Presentation] Proteins that remodel the membrane shape and their physiological functions. (2009)
  • Organizer: IUPS2009
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-31
• [Presentation] EFC/F-BAR domain super family (2009)
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] EFC/F-BAR proteins and the N-WASP-WIP complex bind to membranes to induce membrane-curvature dependent actin polymerization. (2008)
  • Organizer: the 48th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Moscone Center (San Francisco)
  • Year and Date: 2008-12-17
• [Presentation] EFC/F-BARドメインタンパク質FBP17およびToca-1とN-WASP-WIP複合体による膜曲率依存的アクチン重合の誘導 (2008)
  • Organizer: 第31回分子生物学会年会 第81回日本生化学会大会 合同大会(BMB2008)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-10
• [Presentation] 膜曲率によるアクチン重合の制御 (2008)
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-07-01
• [Book] Coupling Membrane Dynamics to Actin Polymerization(1st Edition., 2010, XII, Part 1)(Marie-France (Ed.)) (2010)
  • Author(s): Shiro Suetsugu, Tadaomi Takenawa
  • Total Pages: 300(35-57)
  • Publisher: Actin-based Motility Carlier
• [Remarks] ホームページ等
  • URL: http://www.iam.u-tokyo.ac.jp/suetsugu/
• [Remarks]
  • URL: http://www.suetsugulab.net