Tissue engineering based on reversible modification of bio-oriented scaffolds

2009 Fiscal Year Final Research Report

• Project/Area Number: 20700401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: National Institute for Materials Science
• Principal Investigator: NAKANISHI Jun National Institute for Materials Science, 国際ナノアーキテクトニクス研究拠点, 独立研究者 (60360608)
• Project Period (FY): 2008 – 2009
• Keywords: バイオマテリアル

Research Abstract

In this study, I have developed a method for reversible modification of the surface properties of bio-oriented scaffolds by using a molecule that can be once conjugated but cleaved in response to light. As a proof of concept, I demonstrated a reversible modification of an amino-terminated surface with poly(ethylene glycol) and control of its cell adhesiveness. In addition, I have succeeded in the control of the activity of biological amine and the formation of surface chemical gradients on gold nanoparticles and substrates based on the similar strategy.

Research Products

• [Journal Article] Silane-coupling Agent Having a Photoremovable Succinimidyl Carbonate for Patterning Amines on Glass and Silicon Surfaces with Controlled Surface Densities (2010)
  • Author(s): H. Nakayama, J. Nakanishi, T. Shimizu, Y. Yoshino, S. Kaneko, Y. Horiike, K. Yamaguchi
  • Journal Title: Colloids and Surfaces B 76 Pages: 88-97
  • Peer Reviewed
• [Journal Article] Light-Regulated Activation of Cellular Signaling by Gold Nanoparticles That Capture and Release Amines (2009)
  • Author(s): Jun Nakanishi, Hidekazu Nakayama, Takahiro Shimizu, Haruhisa Ishida, Yukiko Kikuchi, Kazuo Yamaguchi, Yasuhiro Horiike
  • Journal Title: Journal of the American Chemical Society 131 Pages: 3822-3823
  • Peer Reviewed
• [Journal Article] Precise patterning of photoactivatable glass coverslip for fluorescence observation of shape-controlled cells
  • Author(s): J. Nakanishi, Y. Kikuchi, Y. Tsujimura, H. Nakayama, S. Kaneko, T. Shimizu, H. Yokota, K. Yamacughi, Y. Yoshida, T. Takarada, M. Maeda, Y. Horiike
  • Journal Title: Supramolecular chemistry (in press)
  • Peer Reviewed
• [Presentation] 光解離性スクシンイミジルエステルを有する単分子膜基板でのアミン誘導体の表面密度制御 (2009)
  • Author(s): 中山秀一, 中西淳, 清水孝弘, 吉野雄太郎, 岩井秀夫, 金子信悟, 山口和夫, 堀池靖浩
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-18
• [Presentation] 光解離性PEGを修飾したアミノ化表面に基づくケージド基板 (2009)
  • Author(s): 金子信悟, 中西淳, 中山秀一, 吉野雄太郎, 山口和夫, 堀池靖浩
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-17
• [Book] ナノ空間材料の創製と応用 (2009)
  • Author(s): 中西淳(分担執筆)
  • Total Pages: 205-213
  • Publisher: フロンティア出版
• [Patent(Industrial Property Rights)] 光分解性ヘテロ二価性架橋剤 (2009)
  • Inventor(s): 山口和夫, 中西淳
  • Industrial Property Rights Holder: 神奈川大学, 物質・材料研究機構
  • Industrial Property Number: 特願2009-114028
  • Filing Date: 2009-05-08