2009 Fiscal Year Final Research Report
Direct protein analysis from electrophoresis gel using a mid-infrared laser
| Project/Area Number |
20750062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
SUZUKI Sachiko Osaka University, 工学研究科, 助教 (20403157)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 生体分析 / 赤外レーザー / 質量分析 / イオン化 / 蛋白質 / 直接分析 |
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| Research Abstract |
In this study, I performed protein analysis from acrlamide gel after electrophoresis using a Mid-infrared tunable laser. I reported that the IR-MALDI process is influenced by the hydrogen bond around the carboxyl group or residual water. The aclylamide solution has a strong absorption bands at around 6.0μm correstponding to the >C=O stretching vibration mode. I obtained the mass spectrum of insulin ionized from acrylamide gel when the sample was irradiated by the infrared laser in a wavelength range of 5.8-5.9μm.
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