2009 Fiscal Year Final Research Report
Rational strategy of tuning protein-complex function by ligand
| Project/Area Number |
20750133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
MIZUNO Toshihisa Nagoya Institute of Technology, 大学院・工学研究科, 助教 (90345950)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ナノバイオ / 蛋白質 / バイオテクノロジー |
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| Research Abstract |
As a model system of protein-protein interaction, we chose the T7 RNA polymerase-T7 lysozyme pair in this research. By inserting the originally designed coiled-coil protein, which transform its tertiary structure from random coil to coiled-coil structure by the specific ligand peptide, into the T7 lysozyme, we succeeded in tuning the interaction between T7 RNA polymerase and T7 lysozyme by the binding of specific ligand peptide. On the other hand, we also succeeded in constructing the ligand-binding protein, which exhibits a reversible structural alteration following the specific ligand binding. Interestingly, this protein can work even in the in vivo circumstances. By combining this designed ligand-binding protein with various target natural proteins, ligand-binding tuning of protein-protein interactions should be possible in future.
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[Journal Article] Reconstitution of the Light-harvesting (LH1) Complex Using Zinc-substituted Bacteriochlorophyll a and LH1-polypeptides Isolated from the Purple Photosynthetic Bacterium Rhodospirillum rubrum, Together with all-trans Carotenoids2010
Author(s)
K. Nakagawa, T. Nakano, N. Fukui, A. Nakashima, S. Sakai, T. Mizuno, T. Dewa, K. Iida, A.T. Gardiner, R.J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
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Journal Title
Carotenoid Science (in press)
Peer Reviewed
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[Journal Article] Reconstitution of the Light-harvesting 1 (LH1) Complex Using LH1-α and LH-1-β Polypeptides, Separately Isolated from the Purple Photosynthetic Bacterium Rhodospirillum rubrum, Together with Bacteriochlorophyll a and All-trans Carotenoids2010
Author(s)
K. Nakagawa, T. Nakano, N. Fukui, A. Nakashima, S. Sakai, T. Mizuno, T. Dewa, K. Iida, A.T. Gardiner, R.J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
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Journal Title
Carotenoid Science (in press)
Peer Reviewed
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[Journal Article] Carotenoid Specificity During Reconstitution of the Light-harvesting 1 (LH1) Complexes Using LH1-polypeptides Isolated from the Purple Photosynthetic Bacterium Rhodobacter sphaeroides Together with Bacteriochlorophyll a and Carotenoids2010
Author(s)
K. Nakagawa, N. Fukui, T. Nakano, A. Mizuno, A. Nakashima, S. Sakai, T. Mizuno, T. Dewa, K. Iida, A.T. Gardiner, R.J. Cogdell, R. Fujii, H. Hashimoto, M. Nango
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Journal Title
Carotenoid Science (in press)
Peer Reviewed
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[Journal Article] The effect of the side chain length of Asp and Glu on coordination structure of Cu (2+) in a de novo designed protein2009
Author(s)
D. Shiga, D. Nakane, T. Inomata, H. Matsuda, M. Oda, M. Noda, S. Uchiyama, K. Fukui, Y. Takano, H. Nakamura, T. Mizuno, T. Tanaka
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Journal Title
Biopolymer 91
Pages: 907-916
Peer Reviewed
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