Dissection of mammalian mitochondrial protein synthesis system

2009 Fiscal Year Final Research Report

• Project/Area Number: 20770135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: TOMITA Nono (竹内 野乃) The University of Tokyo, 大学院・新領域創成科学研究科, 助教 (80323450)
• Project Period (FY): 2008 – 2009
• Keywords: 翻訳 / ミトコンドリア

Research Abstract

To obtain the detailed insight on the molecular mechanism on mitochondrial translation, we studied the mechanism of ribosome recycling process, utilizing the in vitro translation system, reconstituted by ourselves. We discovered the novel translation factor EF-G2mt/RRF2mt which mediates ribosome recycling together with human mitochondrial RRF (RRFmt).We also studied the pathogenic mechanism for the mutation in EF-Tumt and EF-Tsmt.

Research Products

• [Journal Article] 哺乳類ミトコンドリアにおける新規翻訳因子の発見 (2010)
  • Author(s): 竹内野乃、上田卓也
  • Journal Title: 生化学 ((印刷中) (掲載確定))
• [Journal Article] Spremulli Analysis of the Functional Consequences of Lethal Mutations in Mitochondrial Translational Elongation Factors (2010)
  • Author(s): Kenta Akama, Brooke E. Christian, Christie N. Jones, *Nono Takeuchi, Linda L.
  • Journal Title: BBA (in press (掲載確定))
  • URL: doi:10.1016/j.bbadis.2010.04.003
• [Journal Article] A bacterial elongation factor G homolog exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi (2010)
  • Author(s): Suematsu T, Yokobori SI, Morita H, Yoshinari S, Ueda T, Kita K, *Takeuchi N, Watanabe YI.
  • Journal Title: Mol Microbiol 75 Pages: 1445-1454
• [Journal Article] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis. (2009)
  • Author(s): Tsuboi M., Morita H., Nozaki Y., Akama K., Ueda T., Ito K., Nierhaus KH., *Takeuchi N.
  • Journal Title: Molecular Cell 28 Pages: 502-10
• [Presentation] Dissection of ribosome recycling mechanism in mammalian mitochondrial protein synthesis (2010)
  • Author(s): Nono Takeuchi
  • Organizer: Current Issues of Ribosomal Structure and Function
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2010-04-09
• [Presentation] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis (2010)
  • Author(s): Hiroyuki Morita, Masafumi Tsuboi, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H. Nierhaus, Nono Takeuchi
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-02-01
• [Presentation] 哺乳類ミトコンドリアにおけるリボソーム再生因子の作用機序 (2009)
  • Author(s): 森田寛幸、野崎雄介、壺井将史、上田卓也、○竹内野乃
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-17
• [Presentation] Functional Dissection of Ribosome Recycling Factor (RRF) in mammalian mitochondria: Role of the N-terminal domain of mitochondrial RRF in ribosome recycling. (2009)
  • Author(s): Hiroyuki Morita, Yusuke Nozaki, Masafumi Tsuboi, Takuya Ueda, Nono Takeuchi
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] EF-G2MT IS AN EXCLUSIVE RECYCLING FACTOR IN MAMMALIAN MITOCHONDRIAL PROTEIN SYNTHESIS EMBO (2009)
  • Author(s): Masafumi Tsuboi, Hiroyuki Morita, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H. Nierhaus, Nono Takeuchi
  • Organizer: Conference Series on Protein Synthesis and Translational Control
  • Place of Presentation: Heiderberg, Germany
  • Year and Date: 2009-09-12
• [Presentation] 哺乳類ミトコンドリアにおけるリボソーム再生因子の作用機序 (2009)
  • Author(s): 森田寛幸、野崎雄介、壺井将史、上田卓也、○竹内野乃
  • Organizer: 日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-29
• [Remarks]
  • URL: http://www.k.u-tokyo.ac.jp/news/20090824press.html#top