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2009 Fiscal Year Final Research Report

Analysis of proteins regulating bacterial cell division site

Research Project

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Project/Area Number 20770142
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionUniversity of Toyama

Principal Investigator

OKUNO Takashi  University of Toyama, 大学院・医学薬学研究部(薬学), 准教授 (80411031)

Project Period (FY) 2008 – 2009
Keywords細胞分裂 / ATPase
Research Abstract

We have studied on the Min proteins (MinC,D,E) which regulates position of ceptum ring (FtsZ ring) formation in E.coli. he results of FRET analysis indicated that MinC interacts with FtsZ monomer rather than FtsZ polymer in the solution. It is suggested that interaction between monomeric FtsZ and MinC is important for controlling of FtsZ polymerization by MinC. On the other hand, in vitro analysis of mutant proteins of MinD indicated membrane binding of MinD requires not only ATP binding to nucleotide binding pocket of MinD, but also other factors.

  • Research Products

    (7 results)

All 2010 2009 2008 Other

All Journal Article (2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Comparison of intracellular localization between Nubp1 and Nubp2 utilizing GFP fusion protein.2010

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K.
    • Journal Title

      Mol. Biol. Rep. 37

      Pages: 1165-1168

  • [Journal Article] Direct monitoring of interaction between Escherichia coli proteins., MinC and monomeric FtsZ, in solution.2009

    • Author(s)
      Okuno T., Ogoh M., Tanina H., Funasaki N., Kogure K.
    • Journal Title

      Biol. Pharm. Bull. 32

      Pages: 1473-1475

  • [Presentation] Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.2009

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K., Ueno M.
    • Organizer
      日本分子生物学会第32回年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
  • [Presentation] 新規の平面脂質膜構造体の膜タンパク質機能のイメージングへの応用2009

    • Author(s)
      奥野貴士, 小暮健太朗, 上野雅晴
    • Organizer
      日本薬学会北陸支部121回例会
    • Place of Presentation
      富山
    • Year and Date
      2009-12-05
  • [Presentation] Analysis of interaction between MinC and FtsZ by FRET.2008

    • Author(s)
      奥野貴士, 丸山真由美, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-03
  • [Presentation] FRETを利用したバクテリア分裂制御タンパク質間の相互作用の解析2008

    • Author(s)
      奥野貴士, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      第58回日本薬学会近畿支部大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-10-25
  • [Remarks]

    • URL

      http://www.pha.u-toyama.ac.jp/phypha1/index-j.html

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Published: 2011-06-18   Modified: 2016-04-21  

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