2010 Fiscal Year Final Research Report
Construction of a model for recovery of limb regenerative capacity in non-regenerative animals
| Project/Area Number |
20770168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
YOKOYAMA Hitoshi 東北大学, 大学院・生命科学研究科, 助教 (90455816)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 四肢 / 発生 / Xenopus / 再生医学 / transgenesis |
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| Research Abstract |
In amphibians, Xenopus is advantageous because we can prepare numerous transgenic animals efficiently. I aimed at recovery of limb regeneration in metamorphosed Xenopus, which has only restricted regenerative capacity of a limb. I modified the gene manipulation protocols to achieve effective control of gene expression at the beginning. Then, I examined the role of Wnt/β-catenin signaling in the initiation of limb regeneration and indicated the different mechanisms of limb regeneration initiation in tadpoles and young adults with reference to Wnt/β-catenin signaling. Finally, I found that the activation of Wnt/β-catenin signaling can rescue the loss of regenerative capacity in denervated limb of a young adult frog, which cannot regenerate at all originally.
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