Meiotic transition of germline stem cells and molecular regulation of homologous chromosome recombination

2009 Fiscal Year Final Research Report

• Project/Area Number: 20770177
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: CHUMA Shinichiro Kyoto University, 再生医科学研究所, 助教 (20378889)
• Project Period (FY): 2008 – 2009
• Keywords: 生殖細胞 / 減数分裂 / 発生 / ゲノム

Research Abstract

In this research project, we developed a novel culture system in which an established germline stem cell line derived from mouse spermatogonia can be synchronously induced to enter into meiotic prophase in vitro. This culture system enabled detailed molecular and biochemical analyses on mammalian meiosis such as inductive and inhibitory signaling pathways, their crosstalk and chromatin dynamics during homologous chromosome recombination.

Research Products

• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo T, Suzuki T, Hata K, Martin SL, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai RS, Nakatsuji N, Chuma S
  • Journal Title: Dev Cell. 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: germinal granules in mammals (2009)
  • Author(s): Chuma S, Hosokawa M, Tanaka T, Nakatsuji N
  • Journal Title: Mol Cell Endocrinol. 306 Pages: 17-23
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin (2008)
  • Author(s): Kanatsu-Shinohara M, Takehashi M, Takashima S, Lee J, Morimoto H, Chuma S, Raducanu A, Nakatsuji N, Fassler R, Shinohara T
  • Journal Title: Cell Stem Cell. 3 Pages: 533-542
  • Peer Reviewed
• [Presentation] The roles of tudor genes in mammalian spermatogenesis. (2009)
  • Author(s): Takashi Tanaka, Mihoko Hosokawa, Norio Nakatsuji, Shinichiro Chuma
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: 15th European testis workshop with NAFA annual meeting
  • Place of Presentation: Naantali, Finland
  • Year and Date: 20080502-20080506