Comprehensive analysis of rice DREB1/CBF-type transcription factors

2010 Fiscal Year Final Research Report

• Project/Area Number: 20780006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: 伊藤 裕介 Japan International Research Center for Agricultural Sciences, 生物資源領域, 研究助手 (30399373)
• Project Period (FY): 2008 – 2010
• Keywords: 植物 / 環境ストレス / 発現制御 / 転写因子 / 遺伝子組換え

Research Abstract

The transcription factors DRE (dehydration responsive element) binding protein 1s (DREB1s)/ C-repeat (CRT) binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis thaliana. Rice (Oryza sativa) has 10 genes for DREB1 orthologues, OsDREB1s. We analyzed the 10 OsDREB1 genes comprehensively. Many OsDREB1 genes were induced by cold stress (4℃) specifically, and some OsDREB1 genes were also induced by high-salinity (250mM NaCl) or drought stresses. Although all the OsDREB1s except for OsDREB1D have transactivation activity, there is a difference of DNA binding preference among the 10 OsDREB1s. Overexpression of OsDREB1G which has the highest transactivation activity among the 10 OsDREB1s affected expression of many genes in transgenic rice plants which contain the OsDREB1G controlled under a promoter responsive to an animal hormone, dexamethasone (DEX) and some of the dowmstream genes were also regulated by OsDREB1A. These results indicate that rice has some DREB1 genes and utilizes these genes elaborately according to various stresses.

Research Products

• [Journal Article] The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice. (2010)
  • Author(s): Takasaki, H., Maruyama, K., Kidokoro, S., Ito, Y., Fujita, Y., Shinozaki, K., Yamaguchi-Shinozaki, K., Nakashima, K.
  • Journal Title: Mol Genet Genomics Vol.284 Pages: 173-183
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes. (2010)
  • Author(s): Matsukura, S., Mizoi, J., Yoshida, T., Todaka, D., Ito, Y., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Mol.Genet.Genomics Vol.283 Pages: 185-196
  • Peer Reviewed
• [Journal Article] Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. (2009)
  • Author(s): Nakashima, K., Ito, Y, Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Physiol. Vol.149 Pages: 88-95
  • Peer Reviewed
• [Presentation] イネのDREB1/CBFファミリー遺伝子の網羅的解析 (2010)
  • Author(s): 伊藤裕介, 高崎寛則, 圓山恭之進, 篠崎一雄, 篠崎和子
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 20100300
• [Presentation] Comprehensive analysis of DREB1/CBF family genes involved in abiotic-stress responsive gene expression in rice. (2009)
  • Author(s): Yusuke Ito, Kyonoshin Maruyama, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki.
  • Organizer: 6th International Rice Genetics Symposium
  • Place of Presentation: Manila Hotel (Manila, Phillipine)
  • Year and Date: 20091100
• [Presentation] イネのDREB1/CBF ファミリー遺伝子の網羅的解析 (2009)
  • Author(s): 伊藤裕介, 圓山恭之進, 篠崎一雄, 篠崎和子
  • Organizer: 日本植物生理学会第50回年会
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 20090300
• [Presentation] イネのDREB1ファミリー遺伝子の網羅的解析 (2008)
  • Author(s): 伊藤裕介, 篠崎一雄, 篠崎和子
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学(彦根)
  • Year and Date: 20081000