2010 Fiscal Year Final Research Report
Identification of genes affecting the efficiency of genetic transformation in wheat.
| Project/Area Number |
20780007
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
ABE Fumitaka National Agricultural Research Organization, 作物研究所麦類遺伝子技術研究チーム, 主任研究員 (30370547)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 植物分子育種 / 形質転換 / 網羅的遺伝子発現解析 |
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| Research Abstract |
In order to improve the system of genetic transformation in wheat, gene expression patterns of wheat cultured cells through transformation process were studied. Wheat cultivar "Bobwhite" which is the most suitable cultivar to transform was used as plant material. The change of gene expression levels caused by improved condition of transformation was analyzed using microarray. Expression variation of selected genes was compared with two varieties, Bobwhite and Nourin-61, by RT-PCR.
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