Analysis and application of the availability of an inorganic polyphosphate specific to Mycobacterium tuberculosis

2011 Fiscal Year Final Research Report

• Project/Area Number: 20780066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MORI Shigetarou 国立感染症研究所, 細菌第二部, 主任研究官 (60425676)
• Project Period (FY): 2008 – 2011
• Keywords: 結核菌 / ヌクレオチド / 加リン酸分解 / 遺伝子 / タンパク質

Research Abstract

In this study, the function and structure of a polyphosphate-related protein-Rv2613c-was analyzed. This protein is encoded by Rv2613c, a gene that is found in Mycobacterium tuberculosis H37Rv and has unknown function. The results showed that Rv2613c has diadenosine polyphosphate(Ap_nA) phosphorylase activity. Furthermore, Rv2613c was found to have a tetrameric structure that is unique among enzymes that use Ap_nA as a substrate, and it was found that the formation of the tetrameric structure of Rv2613c is essential for its enzymatic activity.

Research Products

• [Journal Article] Structural insights into the novel diadenosine 5', 5'_P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2011)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Journal of Molecular Biology Volume: 410 Pages: 93-104
  • DOI: DOI:10.1016/j.jmb.2011.04.059
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Acta Crystallographica Section F Volume: 66 Pages: 279-281
  • DOI: DOI:10.1107/S174430910905444X
  • Peer Reviewed
• [Journal Article] Purification and molecular characterization of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Journal Title: Protein Expression and Purification Volume: 69 Pages: 99-105
  • DOI: DOI:10.1016/j.pep.2009.09.010
  • Peer Reviewed
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の活性発現に関わる構造的要因の解明 (2011)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第63回日本生物工学大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2011-09-26
• [Presentation] Structural Insights into the Novel Diadenosine Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv (2011)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-10
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の機能構造相関解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-29
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の構造と機能の相関解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 第93回日本細菌学会関東支部総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-10-21
• [Presentation] Cloning, purification, and molecular characterization of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010)
  • Author(s): Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa
  • Organizer: The 110^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: San Dieeo Convention Center(USA)
  • Year and Date: 2010-05-27
• [Presentation] 結核菌由来新規diadenosine 5', 5'-P^1, P^4-tetraphosphate加リン酸分解酵素の結晶構造解析 (2010)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,荒川宜親
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-03-29
• [Presentation] 結核菌由来新規ヌクレオチド加水分解酵素の機能解析 (2009)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,朴貞玉,荒川宜親
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2009-03-29
• [Presentation] 結核菌由来の新規ヌクレオチド加水分解酵素に関する研究 (2009)
  • Author(s): 森茂太郎,柴山恵吾,和知野純一,朴貞玉,荒川宜親
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-03-13
• [Remarks] (1) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystallographic analysis of diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. Photon Factory Activity Report 2009# 27 Par B(2010), P228
• [Remarks] (2) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystal structure of a novel diadenosine 5', 5'-P^1, P^4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. Photon Factory Activity Report 2010# 28 Par B(2011), P260
• [Remarks] (3) Shigetarou Mori, keigo Shibayama, Jun-Ichi Wachino, and Yoshichika Arakawa. Crystal Structure of a Novel Diadenosine 5', 5'-P^1, P^4-Tetraphosphate Phosphorylase from Mycobacterium tuberculosis H37Rv. PDB ID : 3ANO, 2011年5月18日