Development of technology for specific quantitative detection and removal of Endocrine Disruptors existing in our close environment

2010 Fiscal Year Final Research Report

• Project/Area Number: 20780234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: Ube National College of Technology
• Principal Investigator: ITO Taiji Ube National College of Technology, 疾患ゲノム研究センター, 講師 (60343109)
• Project Period (FY): 2008 – 2010
• Keywords: 環境ホルモン / 環境技術 / 生体機能利用 / バイオテクノロジー / 発現制御

Research Abstract

Endocrine Disruptors are thought to correlate with increase of genital tumors, influence for central nervous system and decrease of the number of sperms. In this study, Nonylphenol existing in our close environment is picked up. The receptor proteins were screened as binding proteins to transcriptional co-activator protein, AIBI, in the presence of Nonylphenol using yeast two-hybrid system. Novel protein NPRI (Nonylphenol receptor 1) was isolated.
Biomolecular interaction assay revealed that NPRI specifically binds to Nonylphenol. NPRI localized mainly in endoplasmic reticulum (ER) under non-stress condition, however, in the presence of Nonylphenol, it partly localized in the nucleus and co-localized with AIBI in LNCap cells.
These results strongly suggested that NPRI, in response to Nonylphenol, would translocate from ER to nucleus to disrupt AIBI function, resulting in endocrine disruption.

Research Products

• [Journal Article] Requiem protein links RelB/p52 and theBrm-type SWI/SNF complex in a noncanonicalNF-kappa B pathway. (2010)
  • Author(s): Tando, T., Ishizaka, A., Watanabe, H.,Ito, T., Iida, S., Haraguchi, T., Mizutani,T., Izumi, T., Isobe, T., Akiyama, T.,Inoue, J.I., Iba, H.
  • Journal Title: J.Biol.Chem. 285 Pages: 21951-21960
  • Peer Reviewed
• [Journal Article] miR-21 gene expression triggered by AP-1is sustained through a double-negativefeedback mechanism. (2008)
  • Author(s): Fujita, S., Ito, T., Mizutani, T.,Minoguchi, S., Yamamichi, N., Sakurai, K., Iba, H.
  • Journal Title: J.Mol.Biol. 378 Pages: 492-504
  • Peer Reviewed
• [Journal Article] Brm transactivates the telomerase reversetranscriptase (TERT) gene and modulatesthe splicing patterns of its transcriptsin concert with p54nrb. (2008)
  • Author(s): Ito, T., Watanabe, H., Yamamichi, N.,Kondo, S., Tando, T., Haraguchi, T.,Mizutani, T., Sakurai, K., Fujita, S.,Izumi, T., Isobe, T., Iba, H.
  • Journal Title: Biochem.J. 411 Pages: 201-209
  • Peer Reviewed
• [Presentation] 内分泌攪乱物質ノニルフェノールの新規受容体タンパク質NPR1の単離と機能解析 (2010)
  • Author(s): 伊藤太二、吉屋愛恵、今橋拓士、木下由紀、林間はるほ、山田恵美、吉岡瞳
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール(鳥取県)
  • Year and Date: 20100528-20100529
• [Presentation] Identification and characterization of a novel receptor NPR1 for Endocrine Disruptor Nonylphenol (2009)
  • Author(s): 伊藤太二、今橋拓士、兼重洋平、中川麻衣、増田宏太
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-11
• [Remarks] ホームページ等
  • URL: http://www.genome.tokushima-u.ac.jp/dmb/DMB/homu.html