2009 Fiscal Year Final Research Report
Structural elucidation of the efficient electron transport between photosynthetic membrane proteins and electron carrier
| Project/Area Number |
20790029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UEDA Takumi The University of Tokyo, 大学院・薬学系研究科, 助教 (20451859)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 構造生物学 / 生化学 / 膜タンパク質 / 核磁気共鳴法 / 光合成名反応 / タンパク質-タンパク質相互作用 / 電子移動反応 / 転移交差飽和法 |
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| Research Abstract |
Plastocyanin (Pc) efficiently proceeds the photosynthetic light reaction by rapidly transport an electron to photosystem I (PSI). In this study, we successfully prepared cadmium-substituted spinach Pc (Cd-Pc), and determined its PSI-binding site by transferred cross-saturation (TCS) experiments. Our study revealed that oxidation of the cupper ion on Pc resulted in conformational change in loop 1, which causes reduction of the affinity between Pc and PSI, and the reduction of the affinity would enable the rapid dissociation of Pc from PSI, which is important for the efficient electron transport.
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[Journal Article] Structural basis of the interaction between chemokine stromal cell-derived factor-1/CXCL12 and its G-protein-coupled receptor CXCR42009
Author(s)
Yutaka Kofuku, Chie Yoshiura, Takumi Ueda, Hiroaki Terasawa, Takahiro Hirai, Sae Tominaga, Masako Hirose, Yoshitake Maeda, Hideo Takahashi, Yuya Terashima, Kouji Matsushima, Ichio Shimada
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Journal Title
J. Biol. Chem. Vol.284
Pages: 35240-35250
Peer Reviewed
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