2009 Fiscal Year Final Research Report
Study on neural differentiation of human mesenchymal stem cells based on glycans
| Project/Area Number |
20790110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
HASHII Noritaka National Institute of Health Sciences, 生物薬品部, 主任研究官 (20425672)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 医薬品化学 / 再生医学 |
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| Research Abstract |
To find molecular markers during neural differentiation of human mesenchymal stem cell (MSC), first, we developed a quantitative glycan profiling using liquid chromatography/mass spectrometry in combination with the isotope tagging method. And then, we conducted differential glycan analysis between MSC and the neural-lineage cells by the quantitative glycan profiling, and demonstrated that glycans can be used as a molecular marker for discriminating undifferentiated MSC from neural-lineage cells.
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[Journal Article] Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry: identification of Lewis x-conjugated glycoproteins in mouse kidney.2009
Author(s)
Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Harazono, A., Kawanishi, T., Yamaguchi, T.
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Journal Title
J. Proteome Res. 8
Pages: 3415-3429
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[Journal Article] Alteration of N-glycosylation in the kidney in a mouse model of systemic lupus erythematosus: relative quantification of N-glycans using an isotope-tagging method.2009
Author(s)
Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Kawanishi, T., Yamaguchi, T.
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Journal Title
Immunology 126
Pages: 336-345
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[Journal Article] Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography and multiple-stage mass spectrometry.2009
Author(s)
Itoh, S., Hachisuka, A., Kawasaki, N., Hashii, N., Teshima, R., Hayakawa, T., Kawanishi, T., Yamaguchi, T.
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Journal Title
Biochemistry 47
Pages: 10132-10154
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[Journal Article] Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry.2008
Author(s)
Harazono, A., Kawasaki, N., Itoh, S., Hashii, N., Matsuishi-Nakajima, Y., Kawanishi, T., Yamaguchi, T.
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Journal Title
J Chromatogr B Analyt Technol Biomed Life Sci. 869
Pages: 20-30
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[Presentation] 糖タンパク質医薬品の単糖試験法の標準化.2010
Author(s)
原園景, 小林哲, 川崎ナナ, 伊藤さつき, 多田稔, 橋井則貴, 石井明子, 荒戸照世, 柳原繁弘, 八木有紀, 古賀明子, 津田祐里子, 木村美紀子, 崎田政志, 北村智, 山口秀人, 三村尚志, 村田芳美, 濱詰康樹, 佐藤貴之, 長束俊治, 掛樋一晃, 木下充弘, 渡部沙木絵, 山口照英
Organizer
日本薬学会第130年会
Year and Date
20100300
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