Mechanisms of KCNQ1 channel modulation by KCNE proteins revealed by ascidian KCNQ1 homologue

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790184
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: NAKAJO Koichi National Institute for Physiological Sciences, 分子生理研究系, 助教 (80390699)
• Project Period (FY): 2008 – 2009
• Keywords: イオンチャネル / カリウムチャネル / 生物物理学 / 生理学 / 電位センサー

Research Abstract

KCNQ1, a voltage-gated potassium channel main subunit, is expressed in various tissues like heart, inner ear and intestine, and also known as a causative gene for cardiac arrhythmia and deafness. The property of this ion channel is largely dependent on its auxiliary subunit, KCNE proteins. In this study, we identified some important amino acid residues for KCNQ1 channel modulation by KCNE proteins by comparing human KCNQ1 gene to KCNQ1 homologue from ascidian Ciona intestinalis

Research Products

• [Journal Article] Identification and characterization of Cs+-permeable K+ channel current in mouse cerebellar Purkinje cells in lobules 9 and 10 evoked by molecular layer stimulation (2010)
  • Author(s): Ishii H, Nakajo K, Yanagawa Y, Kubo Y.
  • Journal Title: Eur J Neurosci (in press)
  • Peer Reviewed
• [Journal Article] Voltage- and [ATP]-dependent gating of the P2X2ATP receptor channel (2009)
  • Author(s): Fujiwara Y, Keceli B, Nakajo K, Kubo Y.
  • Journal Title: J Gen Physiol 133(1) Pages: 93-109
  • Peer Reviewed
• [Journal Article] Dynamic aspects of functional regulation of the ATP receptor channel P2X2 (2009)
  • Author(s): Kubo Y, Fujiwara Y, Keceli B, Nakajo K.
  • Journal Title: J Physiol. 587(Pt22) Pages: 5317-24
  • Peer Reviewed
• [Journal Article] Cooper EC Ion channel clustering at the axon initial segment and node of Ranvier evolved sequentially in early chordates (2008)
  • Author(s): Hill AS, Nishino A, Nakajo K, Zhang G, Fineman JR, Selzer ME, Okamura Y
  • Journal Title: PLoS Genet. 4(12) Pages: e1000317
  • Peer Reviewed
• [Journal Article] Second coiled-coil domain of KCNQ channel controls current expression and subfamily specific heteromultimerization by salt bridge networks (2008)
  • Author(s): Nakajo K, Kubo Y
  • Journal Title: J Physiol. 586 Pages: 2827-40
  • Peer Reviewed
• [Presentation] Stoichiometry of KCNQ1-KCNE1 ion channel complex is flexible and density dependent (2010)
  • Author(s): Koichi Nakajo, Maximilian Ulbrich, Yoshihiro Kubo, Ehud Isacoff
  • Organizer: Biophysical Society 54th Annual Meeting
  • Place of Presentation: モスコーンセンター(サンフランシスコ、米国)
  • Year and Date: 2010-02-21
• [Presentation] KCNQ1チャネル複合体ストイキオメトリーの発現依存的変化 (2009)
  • Author(s): Koichi Nakajo, Maximilian Ulbrich, Yoshihiro Kubo, Ehud Isacoff
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-10-31
• [Presentation] KCNE1 modulates KCNQ1 through specific amino acid residues in the pore region (2009)
  • Author(s): Koichi Nakajo, Atsuo Nishino, Yasushi Okamura, Yoshihiro Kubo
  • Organizer: 国際生理学会IUPS2009
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-07-29
• [Presentation] KCNQカリウムチャネル電位センサードメインのダイナミクスとその制御機構 (2008)
  • Author(s): 中條浩一、久保義弘
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2008-12-03
• [Remarks]
  • URL: http://www.nips.ac.jp/contents/release/entry/2008/07/kcnq.html