Regulation of neuronal functions by a lipid-anchored CaM kinase

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790221
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KIMURA Sayaka (竹本 さやか) The University of Tokyo, 大学院・医学系研究科, 助教 (70372365)
• Project Period (FY): 2008 – 2009
• Keywords: 樹状突起 / CaMK / 脂質修飾

Research Abstract

We previously identified and cloned CLICK-III/CaMKIγ, a novel lipid-anchored neuronal CaM kinase, which we further showed played a critical role in dendritogenesis in cultured cortical neurons. Here, we attempted to investigate the physiological functions of this kinase in mouse tissues in vivo. In order to obtain clues about the neuronal functions associated with CLICK-III/CaMKIγ, we generated a knockout mouse with a view to carrying out histological and behavioral analyses. Furthermore, we established in vivo gene transfer methods to study the impact of CLICK-III and related CaMKI isoforms in regulating fine neuronal morphologies during brain development.

Research Products

• [Journal Article] Control of cortical axon elongation by a GABA-driven Ca2+/calmodulin-dependent protein kinase cascade. (2009)
  • Author(s): Ageta-Ishihara N, Takemoto-Kimura S, Nonaka M, Adachi-Morishima A, Suzuki K, Kamijo S, Fujii H, Mano T, Blaeser F, Chatila TA, Mizuno H, Hirano T, Tagawa Y, Okuno H, Bito H
  • Journal Title: The Journal of neuroscience 29 Pages: 13720-9
  • Peer Reviewed
• [Journal Article] Synaptic activity-responsive element in the Arc/Arg3. 1 promoter essential for synapse-to-nucleus signaling in activated neurons. (2009)
  • Author(s): Kawashima T, Okuno H, Nonaka M, Adachi-Morishima A, Kyo N, Okamura M, Takemoto-Kimura S, Worley PF, Bito H
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 106 Pages: 316-21
  • Peer Reviewed
• [Journal Article] シナプス長期可塑性の分子基盤 (2009)
  • Author(s): 井上昌俊, 川島尚之, 野中美応, 竹本-木村さやか, 奥野浩行, 尾藤晴彦
  • Journal Title: Cognition and Dementia 8 Pages: 177-182
• [Journal Article] 神経疾患と細胞骨格 (2009)
  • Author(s): 竹本-木村さやか, 上田(石原)奈津実, 布施俊光, 上條諭志, 尾藤晴彦
  • Journal Title: 分子細胞治療 8 Pages: 243-248
• [Journal Article] シナプス機能とPSD構築を制御する分子機構 (2008)
  • Author(s): 尾藤晴彦, 野中美応, 布施俊光, 藤井哉, 竹本-木村さやか, 奥野浩行
  • Journal Title: 蛋白質核酸酵素 53 Pages: 418-423
• [Presentation] Control of cortical axon elongation by a GABA-driven Ca2+/calmodulin-dependent protein kinase cascade. (2009)
  • Author(s): 上田(石原)奈津美、竹本-木村さやか、野中美応、安達-森島亜希、水野秀信、平野丈夫、田川義晃、奥野浩行、尾藤晴彦
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸ポートアイランド, Oral and Poster
  • Year and Date: 20091021-20091024
• [Presentation] Activity-dependent regulation of dendritic growth. Neurosci. Res. (2009)
  • Author(s): Takemoto-Kimura S, Ageta-Ishihara N, Nonaka M, Adachi-Morishima A, Suzuki K, Bito H.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 20090916-20090918
• [Presentation] A pivotal role of a CaMKK-Ca2+/calmodulin-dependent protein kinase I cascade in the radial migration of layer 2/3 cortical pyramidal neurons. (2009)
  • Author(s): Takemoto-Kimura S, Adachi-Morishima A, Ageta-Ishihara N, Suzuki K, Nonaka M, Okamura M, Nishimura VY, Kawauchi T, Nakajima K, Okuno H, Bito H.
  • Organizer: 第39回北米神経科学学会年会
  • Place of Presentation: Chicago, USA. Poster
  • Year and Date: 2009-10-19