2009 Fiscal Year Final Research Report
Production of gene Knock-down animal by introducing RNAi into spermatogonial stem cells.
Project/Area Number |
20790302
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Experimental pathology
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Research Institution | Kyoto University |
Principal Investigator |
TAKASHIMA Seiji Kyoto University, 医学研究科, 助教 (40396891)
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Project Period (FY) |
2008 – 2009
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Keywords | 疾患モデル動物 |
Research Abstract |
To assess whether RNAi mediated gene knock-down could be applied to germline stem cells, RNAi construct for EGFP knock-down was transduced to germline stem cells derived from EGFP transgenic mouse. Although lentivirus mediated RNAi transduction suppressed the EGFP expression to some extent in germline stem cells, the efficiency was not enough to produce the knock-down mouse. Meanwhile, by using RNAi technology developed in this project, it was shown that DNA methyl transferase I is indispensable for the survival of germline stem cells in vitro, suggesting that RNAi mediated knockdown is available for the endogenous genes.
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Research Products
(8 results)
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[Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.2009
Author(s)
Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T
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Journal Title
Biology of Reproduction. 81(1)
Pages: 155-164
Peer Reviewed
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