Production of gene Knock-down animal by introducing RNAi into spermatogonial stem cells.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790302
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKASHIMA Seiji Kyoto University, 医学研究科, 助教 (40396891)
• Project Period (FY): 2008 – 2009
• Keywords: 疾患モデル動物

Research Abstract

To assess whether RNAi mediated gene knock-down could be applied to germline stem cells, RNAi construct for EGFP knock-down was transduced to germline stem cells derived from EGFP transgenic mouse. Although lentivirus mediated RNAi transduction suppressed the EGFP expression to some extent in germline stem cells, the efficiency was not enough to produce the knock-down mouse. Meanwhile, by using RNAi technology developed in this project, it was shown that DNA methyl transferase I is indispensable for the survival of germline stem cells in vitro, suggesting that RNAi mediated knockdown is available for the endogenous genes.

Research Products

• [Journal Article] Genetic influences in mouse spermatogonial stem cell self-renewal. (2010)
  • Author(s): Kanatsu-Shinohara M, Ogonuki N, Miki H, Inoue K, Morimoto H, Takashima S, Ogura A, Shinohara T.
  • Journal Title: Journal of Reproduction and Development 56(1) Pages: 145-153
  • Peer Reviewed
• [Journal Article] Phenotypic Plasticity of Mouse Spermatogonial Stem Cells. (2009)
  • Author(s): Morimoto H, Kanatsu-Shinohara M, Takashima S, Chuma S, Nakatsuji N, Takehashi M, Shinohara T.
  • Journal Title: PLoS One 4(11) Pages: e7909
  • Peer Reviewed
• [Journal Article] Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Rascyclin D2 activation. (2009)
  • Author(s): Lee J, Kanatsu-Shinohara M, Morimoto H, Kazuki Y, Takashima S, Oshimura M, Toyokuni S, Shinohara T
  • Journal Title: Cell Stem Cell 5(1) Pages: 76-86
  • Peer Reviewed
• [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. (2009)
  • Author(s): Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T
  • Journal Title: Biology of Reproduction. 81(1) Pages: 155-164
  • Peer Reviewed
• [Journal Article] Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes. (2009)
  • Author(s): Izumi-Yoneda N, Toda A, Okabe M, Koike C, Takashima S, Yoshida T, Konishi I, Saito S, Nikaido T
  • Journal Title: Molecular Human Reproduction 15(1) Pages: 49-57
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on beta1- integrin. (2008)
  • Author(s): Kanatsu-Shinohara M, Takehashi M, Takashima S, Lee J, Morimoto H, Chuma S, Raducanu A, Nakatsuji N, Fassler R, Shinohara T
  • Journal Title: Cell Stem Cell 3(5) Pages: 533-542
  • Peer Reviewed
• [Journal Article] Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells. (2008)
  • Author(s): Kanatsu-Shinohara M, Kato M, Takehashi M, Morimoto H, Takashima S, Chuma S, Nakatsuji N, Hirabayashi M, Shinohara T
  • Journal Title: Biology of Reproduction 79(6) Pages: 1121-1128
  • Peer Reviewed
• [Journal Article] Characterization of laminin isoforms in human amnion. (2008)
  • Author(s): Takashima S, Yasuo M, Sanzen N, Sekiguchi K, Okabe M, Yoshida T, Toda A, Nikaido T
  • Journal Title: Tissue and Cell 40(2) Pages: 75-81
  • Peer Reviewed