2009 Fiscal Year Final Research Report
Control of transgenes with insulators about malaria parasite
| Project/Area Number |
20790326
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Parasitology (including Sanitary zoology)
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
YAHATA Kazuhide Nagasaki University, 熱帯医学研究所, 助教 (40467965)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | マラリア / エピジェネティック / インスレーター |
|---|
| Research Abstract |
The development of transgenic methods is advancing as an analytical technique in malaria research. When an artificially constructed transgene vector is introduced into parasites, gene expression sometimes does not occur at the expected time point. However, a region called the "insulator" is known to be located between neighboring genes on the genome DNA, is inserted between adjacent transgenes using an episomal gene, the transcript interference was suppressed in human cells. To evaluate the activity of insulator in malaria parasite and identify a potential insulator on the genome DNA of malaria parasite, we developed assay system using a malaria parasite.
|
