2009 Fiscal Year Final Research Report
The investigation of cytosolic uptake mechanisms for basic amino acid-rich peptides to the effective drug delivery
| Project/Area Number |
20790404
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied pharmacology
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| Research Institution | Otani Womens University |
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Principal Investigator |
OKUDA Akiko Otani Womens University, 薬学部, 助教 (60454584)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ドラッグデリバリー / 塩基性ペプチド |
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| Research Abstract |
The endocytic pathways has recently been demonstrated in the major cellular uptake of the basic amino acids-rich peptides, including HIV-1 Tat and oligoarginine. But the cellular uptake into the cytosol has not been demonstrated by real time monitoring. In this study, I established the detection system of the peptides into the cytosol using a split Kusabira Green complementation assay. The fluorescence derived from the Kusabira Green could be observed by confocal laser scanning microscopy in whole cell by adding the oligoarginine-Kusabira Green fragment to the cell culture media for 5-8 hours.
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