2009 Fiscal Year Final Research Report
Development of a new and useful method in clinical testing of HDL subfractions
| Project/Area Number |
20790414
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
USUI Shinichi Okayama University, 大学院・保健学研究科, 准教授 (50346417)
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| Project Period (FY) |
2008 – 2009
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| Keywords | HDL / アポリポタンパク質 / ELISA |
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| Research Abstract |
In the present study, I studied an enzyme-linked immunosorbent assay for detecting serum high-density lipoprotein (HDL) subfractions by two different types of antibodies against HDL. Using anti apo E and anti apo A-II antibodies, apoAII-E complex that is contained in HDL particles was qualitatively determined under an optimal analytical condition. I, additionally, tried to detect the complex by fluorescence energy transfer method, but did not detect a significant fluorescent signal.
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