2009 Fiscal Year Final Research Report
Molecular biological analysis of the epitherial-mesenchymal transition in various human renal diseases
| Project/Area Number |
20790599
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
UMEZONO Tomoya Tokai University, 医学部, 講師 (40349345)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 上皮-間葉転換 / insitu ybridization / 腎繊維化 / 免疫組織染色 |
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| Research Abstract |
we performed immunohistochemistry with an anti-Snai1 antibody and quantitative analysis in various renal disease. We performed double staining with vimentin antibody to assess its expression in the tubulointerstitial region. Furthermore, we examined the relationship between the number of Snai1- and/or vimentin-positive cells and various clinical and histopathological parameters at the renal biopsy by linear regression analysis. Snai1 protein was observed in the nuclei of damaged tubular epithelial cells with a flattened appearance. Double staining with vimentin showed that several vimentin-positive tubular epithelial cells had Snai1 positive nuclei. On quantitative analysis, the number of Snai1/vimentin double positive cells in the tubulointerstitial region was increased in DN and IgAN specimens, whereas expression was limited in control specimens. In DN specimens, the number of Snai1/vimentin double positive cells was significantly higher when the disease showed rapid progression than when it showed slow progression. These study showed that Snai1 may contribute to the development of tubulointerstitial fibrosis in DN.
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