2009 Fiscal Year Final Research Report
Research for the localization of DJ-1 on the plasma membrane and functional roles of DJ-1 in membrane trafficking systems
| Project/Area Number |
20790626
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Juntendo University |
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Principal Investigator |
IMAMI Satoshi Juntendo University, 薬学部, 研究員 (80468579)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 神経科学 / 脳・神経 / 脳神経疾患 |
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| Research Abstract |
The aim of the present study is to investigate the precise subcellular distribution of endogenous DJ-1 in mouse brain and several cultured cell lines. In the present study, we found that DJ-1 was collected in the synaptic plasma membrane fractions together with integral membrane proteins. In agreement with this finding, double immunolabelling assay showed that DJ-1-immunoreactivity was highly detected in the membrane compartment of cultured cells and overlapped with integral membrane proteins on the cell surface. The findings strongly suggest that DJ-1 is associated with the plasma membrane in the mouse brain and cultured cells.
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