Establishment of the methods for regeneration of steroidogenic cells from mesencymal stem cells and analysis of its mechanism

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790661
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyushu University
• Principal Investigator: TANAKA Tomoko Kyushu University, 医学部, 研究員 (10380528)
• Project Period (FY): 2008 – 2009
• Keywords: 内分泌学 / ステロイドホルモン / 問葉系幹細胞

Research Abstract

Although we reported that Ad4BP/SF-1 transformed mesenchymal stem cells (MSCs) into steroidogenic cells, Ad4BP/SF-1-converted steroidogenic cells produce both adrenal-type steroids and gonad-type steroids. To clarify the underlying mechanism, we analyzed the expression profile and focused on the several factors up-regulated by Ad4BP/SF-1. Among these factors, Sfrp-1 and Inhbb might be related to Ad4BP/SF-1-mediated differentiation of MSCs into gonad-type steroidogenic cells.

Research Products

• [Journal Article] Dihydrotestosterone suppresses foam cell formation and attenuates atherosclerosis development (2010)
  • Author(s): Qiu Y, Yanase T, Hu H, Tanaka T, Nishi Y, Liu M, Sueishi K, Sawamura T, Nawata H
  • Journal Title: Endocrinology (in press)
  • Peer Reviewed
• [Journal Article] ステロイド産生細胞研究の現状 (2009)
  • Author(s): 柳瀬敏彦、田中智子
  • Journal Title: 日本生殖内分泌学会雑誌 14巻 Pages: 51-53
• [Journal Article] A novel synthetic androgen receptor ligand, S42, works as a selective androgen receptor modulator and possesses metabolic effects with little impact on the prostate (2009)
  • Author(s): Liu M, Yanase T, Tanaka T, Fan W, Nomura M, Kawate H, Okabe T, Takayanagi R, Nawata H
  • Journal Title: Endocrinology 12 Pages: 5606-16
  • Peer Reviewed
• [Journal Article] Methylation of a conserved intronic CpG island of mouse SF-1 is associated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression (2008)
  • Author(s): Shirouhzu H, Okabe T, Gondo S, Tanaka T, Ohe K, Morinaga H, Kawate H, Nomura M, Takayanagi R, Nawata H, Yanase T
  • Journal Title: Biochem Biophys Res Commun 369 Pages: 862-7
  • Peer Reviewed
• [Journal Article] Adipose tissue-derived and bone marrow-derived mesenchymal cells develop into different lineage of steroidogenic cells by forced expression of steroidogenic factor-1 (2008)
  • Author(s): Gondo S, Okabe T, Tanaka T, Morinaga H, Nomura M, Takayanagi R, Nawata H, Yanase T
  • Journal Title: Endocrinology 9 Pages: 4717-25
  • Peer Reviewed
• [Presentation] Regeneration of steroidogenic cells form mesenchymal stem cells (2010)
  • Author(s): Tomoko Tanaka
  • Organizer: 14th International Congress of Endocrinology Official Satellite Symposia
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-30
• [Presentation] Effects of Sfrp-1 and Inhibin beta B on SF-1 mediated differntiation of mesenchymal stem cells into steroidogenic cells (2010)
  • Author(s): Tomoko Tanaka
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-28
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: 第17回日本ステロイドホルモン学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-14
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: アンドロロジー学会第28回学術大会
  • Place of Presentation: 富山
  • Year and Date: 2009-07-03
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/interna5/index.html