2009 Fiscal Year Final Research Report
Expressional control of p27Kip1 in neuronal progenitor cells in vivo
| Project/Area Number |
20790744
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2009
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| Keywords | 小児神経学 / 神経前駆細胞 / 大脳皮質発生 / 分化誘導 / 細胞周期 / p27Kip1 / マウス |
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| Research Abstract |
p27^<Kip1>, cell cycle regulatory protein, play a critical role in differentiation of neuronal progenitor cells (NPCs). A purpose of this research was to indentify transcriptional factors regulating p27^<Kip1> expression in NPCs during neocortical development. In binding assay, a signal was detected by using biotinylated oligonucletides of p27^<Kip1> 5' UTR promoter sequence together with nuclear extract from NPCs, indicating that DNA binding factor(s) was bound to the probe. However, in the samples with anti-FOXO1a/3a antibodies, alteration of signal mobility was not observed. Taken together, DNA binding factor(s) is not identified as FOXO1a/3a as previously speculated.
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