2010 Fiscal Year Final Research Report
Analysis of the drug sensitivity and the genetic alteration of melanoma
Project/Area Number |
20790808
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Dermatology
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Research Institution | Sapporo Medical University |
Principal Investigator |
HIDA Tokimasa Sapporo Medical University, 医学部, 助教 (90464487)
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Project Period (FY) |
2008 – 2010
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Keywords | 癌 |
Research Abstract |
The purpose of this study was to establish a primary culture system of melanoma cells which derived from surgical samples of melanoma tissues, mainly from primary acral lentiginous melanoma. As a result, we obtained surgical samples from 19 patients, from which three lines of melanoma culture were obtained. Fresh frozen tissue samples were also collected from the same patients to examine melanoma-related genes and their expression. Hot spot mutations were not detected in N-ras, BRAF or c-kit. This system will allow us to examine the correlation between the behavior of melanoma cells, especially of their drug resistance, and their genetic profile, and to choose effective chemotherapeutic drugs for individual melanoma patients.
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Research Products
(6 results)
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[Journal Article] Agouti protein, mahogunin, and attractin in pheomelanogenesis and melanoblast-like alteration of melanocytes : a cAMP-independent pathway.2009
Author(s)
Hida T, Wakamatsu K, Sviderskaya EV, Donkin AJ, Montoliu L, Lynn Lamoreux M, Yu B, Millhauser GL, Ito S, Barsh GS, Jimbow K, Bennett DC
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Journal Title
Pigment Cell Melanoma Res 22
Pages: 623-634
Peer Reviewed
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