2009 Fiscal Year Final Research Report
Allograft Rejection by Nature Immunity which Consist of Allograft Induced Macrophages
| Project/Area Number |
20791129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
NOMI Hayahito Osaka Medical College, 医学部, 助教 (80418938)
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| Project Period (FY) |
2008 – 2009
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| Keywords | マクロファージ / 同種異型移植 / 急性拒絶 / マウス / トラニラスト |
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| Research Abstract |
At first, we analyzed in tumor cells of allogeneic transplantation models, because it turned out to require more time beyond our expectation to achieve the clear result of suppressing macrophages from allogeneic organ transplantation mouse models. We have already reported that 3 × 10^6 cells Meth A fibro-sarcoma (MA cells) which were allo-transplantated into peritoneal space of a C57BL/6 mouse completely rejected in about 14 days. We have also reported that allograft-induced macrophages (AIM) were effective mainly as cellular disorder of peritoneal infiltrating which were activated in this rejection. MA cells have been known to be CTL resistant cells. In this study, we used GFP transgenic C57BL/6 mice (GFP-mice) for the donors and we finally succeeded in videotaping that the GFP-AIM were biting off the co-cultured allogeneic MA cells. We were sure that the bited off allo-MA cells from this achievement. But, we now have to solve the critical problems whether biting off abilities of the AIM are exert toward allogeneic or neoplastic changes of MA. Now we are examining the ability of AIM derived from PEC to solve this problem, after removing some AIM cells which can respond to neoplastic changes. In addition, we are investigating time course of fluorescence shift of the cells PEC drive adherent cells mainly consist from GFP-AIM with or without various concentration of some of agents influence to macrophage, like as Tranilast(3μM~300μM). We, however, have not found out critical effect of it yet. Next, we will try it in activation phase of AIM.
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