2009 Fiscal Year Final Research Report

Generating Synthetic Anti-HPV18 E7 Peptide

Research Project

Project/Area Number	20791167
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Obstetrics and gynecology
Research Institution	Keio University
Principal Investigator	ONO Akiko Keio University, 医学部, 研究員(非常勤) (70383883)
Project Period (FY)	2008 – 2009
Keywords	癌 / ウイルス / タンパク質
Research Abstract	Quantitative binding assay system to investigate the interaction between HPV18 E7 and RB was constructed as expression plasmids. Peptide construct including LxCxE motif showed a certain but labile amount of inhibitory effect .Adjustment of the peptide by adding a pentamerization domain showed stronger inhibitory effect. Cell viability assay did not show significant difference due to poor plasmid transfection efficiency. Adenovirus vectors expressing the pentamerized peptides were constructed for improvement.

(2 results)