2009 Fiscal Year Final Research Report
Identification of feeder cell surface factor that inhibit epithelial cell differentiation
| Project/Area Number |
20791272
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2009
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| Keywords | 眼発生 / 再生医学 |
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| Research Abstract |
Fibroblasts, especially mouse cell line 3T3, support clonal growth of epithelial cells via direct cell-cell contact. We compared gene expression profile between 3T3 and less-colony supportive corneal fibroblasts, and found abundant expression of Dlk1 in 3T3. However, overexpression or inhibition of Dlk1 in feeder cells did not affect on the epithelial colony supporting ability. Although Dlk1 is highly expressed in 3T3, Dlk1 may not be related to the feeder layer activity.
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