2010 Fiscal Year Final Research Report

The role of Th17 cell and IL-17 in the aggressive periotonditis

Research Project

Project/Area Number	20791625
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Periodontal dentistry
Research Institution	Aichi Gakuin University
Principal Investigator	TANAKA Shigehisa Aichi Gakuin University, 歯学部, 講師 (30367619)
Project Period (FY)	2008 – 2010
Keywords	歯周免疫機能学 / T細胞 / インターロイキン17
Research Abstract	GCF was gathered from the patient diagnosed aggressive periodontitis, and the IL-17 density was measured. But all samples were not detected. The result was similar though it had measured in consideration of the method of gathering GCF. To confirm the existence of IL-17 in the local of gingival, The expression of IL-17mRNA was recognized in gingiva with real-time PCR. Next, It was confirmed that the THP-1 cell produced IL-12 and IL-23 stimulated with LPS. The monocytes isolated from the peripheral blood stimulated with E.coli LPS.IL-12 and IL-23 was measured of in the culture supernatant. Though both cytokines were able to detected, there were no significant difference between the aggressive periodontitis patient and healthy group. It is necessary to be going to increase the number of samples in the future and to examine it.

(3 results)