2009 Fiscal Year Final Research Report
Analysis of information processing in visual cortex of awake rats with in vivo functional calcium imaging
| Project/Area Number |
20800077
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KIMURA Rie The Institute of Physical and Chemical Research, 認知判断モデル連携ユニット, 研究員 (60513455)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 一次視覚野 / カルシウムイメージング / 二光子レーザー走査型顕微鏡 / 方位選択性 / 個体動物 / 無麻酔 / 脳の状態 / 出入力相関 |
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| Research Abstract |
I established a new experimental system to apply in vivo two-photon functional calcium imaging to adult rats in the unanesthetized/anesthetized, head-restrained condition. Using this system, I recorded responses in the visual cortex by visual stimulation. I used pigmented VGAT-Venus transgenic rats, in which GABAergic neurons express a fluorescent protein, and distinguished between inhibitory and excitatory neurons. During the recording, ECoG, the pupil of the eye, and behavior were monitored. I examined orientation selectivity, direction selectivity, and maximum responses, and revealed different properties between an unanesthetized and anesthetized condition.
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