Functional analyses of DNA polymerases in nucleotide excision repair

2009 Fiscal Year Final Research Report

• Project/Area Number: 20810021
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagasaki University
• Principal Investigator: OGI Tomoo Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (80508317)
• Project Period (FY): 2008 – 2009
• Keywords: DNA修復 / ヌクレオチド除去修復

Research Abstract

Nucleotide excision repair (NER) removes the UV photolesions from DNA. Compromised NER activity causes xeroderma pigmentosum, a skin cancer predisposition genetic disorder. The early step of NER is well established, whereas the later step, which involves repair synthesis and nick ligasion, is not well understood. Here we demonstrate that three DNA polymerases coordinately role in the NER repair synthesis.

Research Products

• [Journal Article] Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells (2010)
  • Author(s): 荻朋男(責任著者), S. Limsirichaikul, R. Overmeer, M. Volker, K. Takenaka, R. Cloney, Y. Nakazawa, A. Niimi, Y. Miki, N. Jaspers, L. Mullenders, S. Yamashita, M. Fousteri, A. Lehmann
  • Journal Title: Molecular Cell 37 Pages: 714-27
  • Peer Reviewed
• [Journal Article] A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives (2010)
  • Author(s): Nakazawa, Y., S. Yamashita, A. Lehmann, 荻朋男(責任著者)
  • Journal Title: DNA Repair 9 Pages: 506-16
  • Peer Reviewed
• [Journal Article] A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU) (2009)
  • Author(s): Limsirichaikul, S., A. Niimi, H. Fawcett, A. Lehmann, S. Yamashita, 荻朋男(責任著者)
  • Journal Title: Nucleic Acids Research 37 Pages: 1-10
  • Peer Reviewed
• [Patent(Industrial Property Rights)] 損傷DNA修復物質のスクリーニング方法 (2010)
  • Inventor(s): 荻朋男, 他2名
  • Industrial Property Rights Holder: 長崎大学
  • Filing Date: 2010-01-28
  • Overseas
• [Patent(Industrial Property Rights)] 損傷DNA修復物質のスクリーニング方法 (2009)
  • Inventor(s): 荻朋男, 他2名
  • Industrial Property Rights Holder: 長崎大学
  • Industrial Property Number: 日本国特許出願特願2009-172521
  • Filing Date: 2009-07-23