2009 Fiscal Year Final Research Report

Development of a simple analytical method for the determination of blood level of antifungal drugs and a genetic polymorphism analysis for individual treatment

Research Project

Project/Area Number	20890244
Research Category	Grant-in-Aid for Young Scientists (Start-up)
Allocation Type	Single-year Grants
Research Field	Medical pharmacy
Research Institution	Nigata University of Phermacy and Applied Life Sciences
Principal Investigator	SHIMOEDA Sadahiko Nigata University of Phermacy and Applied Life Sciences, 薬学部, 准教授 (40515087)
Research Collaborator	YAMATO Susumu 新潟薬科大学, 薬学部, 教授 (60057370) NAKAGAWA Saori 新潟薬科大学, 薬学部, 助教 (30410228)
Project Period (FY)	2008 – 2009
Keywords	カラムスイッチングHPLC法 / テーラーメイド治療 / 抗真菌薬 / サイクリングプローブ法 / 遺伝子変異 / Poor Metabolizer / 口腔粘膜細胞 / 毛髪
Research Abstract	A simple analytical method using column-switching has been developed for the determination of blood level of antifungal drugs, vorivonazole (VRCZ) and micafungin. The method developed here has successfully applied for the patient samples, and results showed approximately 25% of the patients had a blood concentration trough value exceeding 4.5μg/mL, at which liver dysfunction may occur, suggesting that the measurement of blood VRCZ concentrations is clinically important for preventing side effects. And a convenient method using cycling probe technique has been developed for the analysis of a genetic polymorphism of drug metabolizing enzyme, CYP2C19.