Novel role of Golgi apparatus for non-cell autonomous events

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H00467
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Shimizu Shigeomi 東京医科歯科大学, 難治疾患研究所, 教授 (70271020)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: GOMED / ゴルジ体 / オートファジー

Outline of Final Research Achievements

GOMED is a cellular function in which trans-Golgi membranes are greatly deformed to encapsulate proteins to be degraded and subsequently fuse with lysosomes to degrade their contents. In the "Elucidation of GOMED execution mechanism," we identified the Wipi3 molecule as the executor molecule and found that phosphorylation of Ulk1 is important in the initial reaction. In "Visualization of GOMED," we developed a new GOMED analysis method, the FLAD method. In "Physiological Functions of GOMED," we found that GOMED contributes to the maintenance of cerebellar cell functions.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

ゴルジ体はこれまで、小胞体から運ばれてきた分子に、糖鎖修飾、リン酸化修飾などの適切な翻訳後修飾を加えることによって、各分子を細胞外、細胞膜、リソソームなどに適切に運搬するオルガネラとして考えられてきた。しかしながら、研究代表者は、Golgi-mediated degradation pathway (GOMED)と命名した細胞機能により、ゴルジ体が、周囲の環境に応じて、細胞外分泌の分泌量や細胞膜レセプターの種類や量を巧妙に調節していることを見出した。