2023 Fiscal Year Final Research Report
Physicochemical factors required for the maintenance of genetic information in clinical cancer and their application to personalized cancer care
| Project/Area Number |
20H00665
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石井 秀始 大阪大学, 大学院医学系研究科, 招へい教授 (10280736)
井上 正宏 京都大学, 医学研究科, 特定教授 (10342990)
西原 広史 慶應義塾大学, 医学部(信濃町), 教授 (50322805)
片山 量平 公益財団法人がん研究会, がん化学療法センター 基礎研究部, 部長 (60435542)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | がん細胞 / 三次元培養 / 物理化学的因子 / 生物学的因子 / 遺伝子発現 / 組織工学 / 薬物感受性 |
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| Outline of Final Research Achievements |
In this study, we focused on the extracellular matrix of the stromal tissue surrounding cancer and established an in vitro three-dimensional culture method in which physicochemical and biological factors were controlled to elucidate the factors necessary for the maintenance of genetic information.
They found that gene expression was maintained for up to three passages by optimizing conditions such as the elastic modulus of the stroma and the concentration of vascular endothelial cell growth factor. In addition, they established a 96-well three-dimensional culture and conducted drug efficacy tests of anticancer agents and molecular-targeted drugs, and confirmed that it is possible to determine the response to anticancer agents. In addition, the possibility that the same approach can be applied to patient primary pancreatic cancer cells was confirmed, which has been investigated using colon cancer cells.
Based on the above, we were able to carry out this study as planned.
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| Free Research Field |
バイオマテリアル
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果より、がん微小環境を制御する間質組織の物理化学的および生物学的因子がどのようにがん細胞の性質維持に重要であるか、遺伝子情報の維持に必要な要因を明らかにすることができた。また、動物実験との比較により薬剤感受性試験に応用できることが確認されたため、がん個別化医療への応用可能性が示された。2023年度より臨床研究を開始するに至ったことは、社会的意義が極めて大きいことを示している。大腸癌以外の癌種への展開も期待される。以上より、本研究で得られた成果は、社会的にも産業的にも有意義であると考えられる。
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