High-quality Cell Construction System by Integrating massively parallel intranuclear delivery and screening technology

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02115
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 20020:Robotics and intelligent system-related
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Nagai Moeto 豊橋技術科学大学, エレクトロニクス先端融合研究所, 教授 (00580557)
• Co-Investigator(Kenkyū-buntansha): 沼野 利佳 豊橋技術科学大学, エレクトロニクス先端融合研究所, 教授 (30462716) ; 石田 忠 東京工業大学, 工学院, 准教授 (80517607)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: オプトポレーション / 細胞スクリーニング / ナノ秒パルスレーザ / 多点光照射 / 光硬化性ゲル

Outline of Final Research Achievements

Various optical absorbers were prepared on substrates. Cells were cultured on the substrates. The entire device was then scanned over its entire surface with a laser to improve the optoporation of the cells. The laser output change was automated by using a motorized wheel equipped with an ND filter. The output power of the laser that would yield the optimum introduction rate when continuously irradiated was investigated.
An automated cell encapsulation system was developed for parallel single-cell screening. The desired single cells were selected using an image analysis tool and converted to the required photopolymerization pattern. Suspended Hela cells were encapsulated in gelatin methacrylate (GelMA) hydrogels and successfully encapsulated by cross-linking the surrounding hydrogel by multiple light irradiation.

Free Research Field

バイオマイクロシステム

Academic Significance and Societal Importance of the Research Achievements

機能を喪失した組織への再生医療，難病への細胞治療といった先進医療の普及が渇望されている。この普及を妨げる原因は，従来の細胞の機能改変・選別技術における低質(機能が不均一で不安定)細胞の混入である。そこで本研究では，(A)超並列核内デリバリと(B)スクリーニング技術を中核として，10の6乗個レベルで高品質 (機能が均一かつ安定)の細胞を獲得するシステムの開発と学術体系の確立を最終目標とした。本研究を通じて，細胞改変・選別後の低質細胞の混入を防ぎ，同時に効率的な再生医療・細胞医療用品質の細胞獲得を可能とする意義がある。