Mechanical processing platform for single cells and single molecules using optically-driven nanotools

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 20020:Robotics and intelligent system-related
• Research Institution: Kagawa University
• Principal Investigator: TERAO KYOHEI 香川大学, 創造工学部, 准教授 (80467448)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ナノ加工 / 1細胞解析 / 1分子計測 / 生体分子加工 / マイクロ流体デバイス

Outline of Final Research Achievements

The objective of this study is to realize mechanical processes, such as cutting and assembling, on individual cells and molecules. This will enable us to disassemble and analyze cells and molecules, leading to a better understanding of biological phenomena. In addition, we can reassemble cells to develop biosamples that are beneficial for drug development and regenerative medicine. To achieve this, we have developed an optically-driven nano-tool that allows for precise induction of enzyme and antibody reactions. Additionally, we have developed technologies for working on a single cell or molecule using a microfluidic worktable. This allows us to position and secure the cell or molecule being processed.

Free Research Field

ナノマイクロ工学

Academic Significance and Societal Importance of the Research Achievements

本研究成果として開発した要素技術を統合することで、将来「１個の細胞・１個の生体分子を望み通りに捕捉し、解析する」「１個の細胞に物質を導入し性質を改変する」、「細胞を１個１個望み通りに組み立て、臓器を作製する」「１個の生体分子を望み通りに切断する」、といった機械加工に相似した操作を細胞と分子に対して1個の解像度で実現することに近づくことが期待される。それにより、1細胞・1分子解析への応用や、有用な細胞サンプルの調製に繋がる。