Cellular functional analysis with super-multiplexed fluorescence/Raman imaging

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02650
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 30020:Optical engineering and photon science-related
• Research Institution: The University of Tokyo
• Principal Investigator: Ozeki Yasuyuki 東京大学, 大学院工学系研究科(工学部), 教授 (60437374)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 超多重イメージング / 誘導ラマン散乱 / ラマンプローブ

Outline of Final Research Achievements

We have developed a fluorescence and stimulated Raman scattering (SRS) integrated microscope to enable simultaneous observation of high-speed Raman image with SRS and fluorescence. Using this system, it was demonstrated that imaging in four Raman colors and four fluorescence colors, a total of eight colors, could be performed in just 133 ms. Furthermore, various biological samples stained with Raman probes were observed, and imaging analysis related to the interaction and dynamics of various cellular organelles, and their co-localization was conducted. Through these efforts, we have established a cellular functional analysis method using ultra-multicolor fluorescence and Raman imaging.

Free Research Field

バイオイメージング

Academic Significance and Societal Importance of the Research Achievements

本研究では細胞内を構成する様々な要素を同時に観察する技術を実現するために、蛍光顕微鏡と高速ラマン顕微鏡を統合する新しい生体観察システムを開発するとともに、本システムを用いて、細胞内の8種の構成要素を30秒程度で観察し、細胞内の様々な構成要素の動きや相互作用を詳細に調べました。本研究成果は、生命の仕組みを明らかにするための新しい生体観察手法を提供するものであり、将来的に生命科学や医学に新しい知見をもたらすことが期待されます。