2023 Fiscal Year Final Research Report

Development of breeding materials for reducing aluminum content in tea leaves by genome-wide association analysis

Research Project

PDF

Project/Area Number	20H02886
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 38010:Plant nutrition and soil science-related
Research Institution	Shizuoka University
Principal Investigator	IKKA Takashi 静岡大学, 農学部, 准教授 (90580647)
Co-Investigator(Kenkyū-buntansha)	廣野祐平国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, グループ長補佐 (10391418) 森田明雄静岡大学, その他部局等, 理事 (20324337) 川木純平静岡県農林技術研究所, 静岡県農林技術研究所, 主任研究員 (60881539) 山下寛人静岡大学, 農学部, 助教 (70915488) 古川一実沼津工業高等専門学校, 物質工学科, 准教授 (90353151)
Project Period (FY)	2020-04-01 – 2024-03-31
Keywords	チャ / アルミニウム / ゲノムワイド関連解析 / ゲノミック予測
Outline of Final Research Achievements	In this study, to understand the fundamental mechanisms controlling the aluminum (Al) content in tea leaves, we conducted a thorough investigation into the relationship between Al content in tea leaves and various tea genetic resources. Using statistical genetic methods, we attempt to identify important factors associated with low Al tea. We performed SNPs genotyping using ddRAD-seq and successfully constructed high-density DNA marker information. Using this SNPs information, we established a research platforms for genomic studies in tea plant. Furthermore, through detailed physiological assays, we discovered that the Al content in tea leaves is likely regulated by the binding capacity of Al in the cell wall, which may serve as the rate-limiting factor.
Free Research Field	植物栄養学
Academic Significance and Societal Importance of the Research Achievements	チャにおいてGP技術が確立できたことで，茶樹の品種改良の効率化が大いに期待できる．つまり，機能性成分以外の重要な農業形質，例えば収量性，病害虫抵抗性，早晩性の予測もDNA情報のみで可能となる．また，茶葉中のAl含量低減に向けた基礎知見を構築でき，これらの知見は植物の酸性土壌耐性機構の全容解明に貢献する．木本系の世代促進技術の改善は憂慮すべきだが，生産者や育成者が品質成分等をオーダーメイドできる育種プラットフォームを確立することで，国際的に注目を集める「日本茶」に新たな付加価値を与えることができ，消費・輸出の拡大など茶業界への多大な貢献が期待できる．