Functional analysis of a solanaceae plant-specific secreted protein that suppresses infection by a wide range of pathogenic oomycetes

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H02985
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Nagoya University
• Principal Investigator: Takemoto Daigo 名古屋大学, 生命農学研究科, 教授 (30456587)
• Co-Investigator(Kenkyū-buntansha): 近藤 竜彦 名古屋大学, 生命農学研究科, 講師 (30362289) ; 竹本 愛子 (田中愛子) 名古屋大学, 生命農学研究科, 研究員 (90464148)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: ナス科植物 / ジャガイモ疫病菌 / 分泌ペプチド / 抗菌性

Outline of Final Research Achievements

Potato late blight is the most important disease of potato, one of the four major crops in the world. We isolated the secreted protein gene SAR8.2m from Nicotiana benthamiana (a model plant of the Solanaceae plants) as an essential gene for resistance to the potato late blight pathogen, Phytophthora infestans. In this study, we determined the structure of the matured peptide of SAR8.2m and found that the synthesized SAR8.2m peptide inhibited spore germination and mycelial elongation of pathogens including potato late blight, gray mold and tomato wilt. In addition, potato transformants expressing SAR8.2m showed enhanced disease resistance, indicating that SAR8.2m plays a central role in non-host resistance of Solanaceae plants.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

本研究では、ナス科植物由来のペプチドが病原性卵菌（ジャガイモ疫病菌）だけではなくは病原性糸状菌（灰色かび病菌およびトマト萎凋病菌）へも抗菌性を示すことが示された。非宿主抵抗性の決定因子として発見された抗菌ペプチドの例はこれまでなく学術的に新規性が高い。また、人工合成できる抗菌ペプチドが、様々な植物病原菌の伸長を阻害する活性を示すことが明らかとなったことから、環境負荷の少ない植物病害の制御資材としての活用も期待される。